Wang Yingjun, Ke Yu, Ren Li, Wu Gang, Chen Xiaofeng, Zhao Qichun
Biomaterial Research Institute, College of Material Science and Engineering, South China University of Technology, Guangzhou, China.
J Biomed Mater Res A. 2009 Mar 1;88(3):616-27. doi: 10.1002/jbm.a.31858.
Covalent immobilization of collagen onto poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) film was achieved to improve its cell compatibility. Amide groups photografted on PHBV films were initially converted into amine groups through Hofmann degradation and collagen was then chemically bonded to amine groups, consequently forming the amide, amine, and collagen-modified PHBV. The structures of these modified PHBV films were confirmed by ATR-FTIR, XPS, and SEM analyses. Compared with that of PHBV film, surface wettability of the modified PHBV films enhanced remarkably. In particular, water contact angle of the collagen-modified PHBV film decreased from 65.0 degrees to 2.1 degrees within 130 s. Sheep chondrocytes cultured on PHBV and modified PHBV films were evaluated by cell adhesion test, MTT assay, and morphological observation under SEM. Results showed that the collagen-modified PHBV film had better cell adhesion and proliferation than other modified PHBV films and PHBV film. Chondrocytes on the collagen-modified PHBV film adhered through filopodia, spread by cytoplasmic webbing, and formed cells layer earlier than other modified ones, indicating that the collagen-modified PHBV is a promising biomaterial for cartilage tissue engineering.
通过将胶原蛋白共价固定在聚（3-羟基丁酸酯-co-3-羟基戊酸酯）（PHBV）薄膜上，以改善其细胞相容性。最初通过霍夫曼降解将光接枝在PHBV薄膜上的酰胺基团转化为胺基团，然后将胶原蛋白化学键合到胺基团上，从而形成酰胺、胺和胶原蛋白改性的PHBV。通过ATR-FTIR、XPS和SEM分析证实了这些改性PHBV薄膜的结构。与PHBV薄膜相比，改性PHBV薄膜的表面润湿性显著提高。特别是，胶原蛋白改性的PHBV薄膜的水接触角在130秒内从65.0度降至2.1度。通过细胞粘附试验、MTT法和SEM下的形态观察对在PHBV和改性PHBV薄膜上培养的绵羊软骨细胞进行了评估。结果表明，胶原蛋白改性的PHBV薄膜比其他改性PHBV薄膜和PHBV薄膜具有更好的细胞粘附和增殖能力。胶原蛋白改性的PHBV薄膜上的软骨细胞通过丝状伪足粘附，通过细胞质网扩展，并比其他改性薄膜更早地形成细胞层，这表明胶原蛋白改性的PHBV是一种有前途的用于软骨组织工程的生物材料。
