Hosokawa T
Department of Surgery, Tokyo Women's Medical College, Daini Hospital, Japan.
Nihon Geka Gakkai Zasshi. 1991 May;92(5):577-86.
To elucidate the mechanism of tumor reduction and local immune response after administration of OK-432 into the lesions of breast cancer, patients were given intratumoral injection of OK-432 before surgery. And precise identification of infiltrative lymphocytes was performed. The subjects were 25 patients with primary breast cancer. These patients were randomly divided into the following 3 groups: 10 patients given intratumoral injection of OK-432, 5 patients given intratumoral injection of physiological saline and 10 patients give no treatment. After operation frozen specimens of each tumor were subjected to simple immunohistochemical staining with Leu 2a, Leu 3a and Leu 7, and to immunohistochemical double staining with combination of Leu 2a x Leu 15 and Leu 3a x Leu-DR. Examination of infiltrative lymphocytes in the tumor revealed the positive rate was 100% for Leu 2a + cells (strongly positive, 70%), 90% for Leu 3a + cells (strongly positive, 70%) and 90% for Leu 7 + cells (strongly positive, 0%) in patients given intratumoral injection of OK-432. Double staining showed cytotoxic T cells and helper T cells were predominant among Leu 2a + cells and Leu3a + cells, respectively. Cytotoxic T cells, helper T cells and NK cells were induced at the tumor site after intratumoral injection of OK-432, suggesting the antitumor immune response of these cells is involved in tumor reduction following local administration of OK-432.
为阐明将OK-432注射到乳腺癌病灶后肿瘤缩小及局部免疫反应的机制，在手术前对患者进行瘤内注射OK-432，并对浸润淋巴细胞进行精确鉴定。研究对象为25例原发性乳腺癌患者。这些患者被随机分为以下3组：10例接受瘤内注射OK-432，5例接受瘤内注射生理盐水，10例不接受治疗。术后，对每个肿瘤的冰冻标本进行Leu 2a、Leu 3a和Leu 7的简单免疫组化染色，以及Leu 2a×Leu 15和Leu 3a×Leu-DR组合的免疫组化双重染色。肿瘤浸润淋巴细胞检查显示，瘤内注射OK-432的患者中，Leu 2a +细胞阳性率为100%（强阳性占70%），Leu 3a +细胞阳性率为90%（强阳性占70%），Leu 7 +细胞阳性率为90%（强阳性占0%）。双重染色显示，Leu 2a +细胞和Leu3a +细胞中分别以细胞毒性T细胞和辅助性T细胞为主。瘤内注射OK-432后，肿瘤部位诱导出细胞毒性T细胞、辅助性T细胞和NK细胞，提示这些细胞的抗肿瘤免疫反应参与了局部注射OK-432后的肿瘤缩小过程。
