Klyen Blake R, Armstrong Julian J, Adie Steven G, Radley Hannah G, Grounds Miranda D, Sampson David D
The University of Western Australia, School of Electrical, Electronic and Computer Engineering, Optical+Biomedical Engineering Laboratory, 35 Stirling Highway, Crawley, Western Australia 6009, Australia.
J Biomed Opt. 2008 Jan-Feb;13(1):011003. doi: 10.1117/1.2870170.
Three-dimensional optical coherence tomography (3D-OCT) is used to evaluate the structure and pathology of regenerating mouse skeletal muscle autografts for the first time. The death of myofibers with associated inflammation and subsequent new muscle formation in this graft model represents key features of necrosis and inflammation in the human disease Duchenne muscular dystrophy. We perform 3D-OCT imaging of excised autografts and compare OCT images with coregistered histology. The OCT images readily distinguish the necrotic and inflammatory tissue of the graft from the intact healthy muscle fibers in the underlying host tissue. These preliminary findings suggest that, with further development, 3D-OCT could be used as a tool for the evaluation of small-animal muscle morphology and pathology, in particular, for analysis of mouse models of muscular dystrophy.
首次使用三维光学相干断层扫描（3D-OCT）来评估再生小鼠骨骼肌自体移植的结构和病理学。在这个移植模型中，肌纤维死亡并伴有相关炎症，随后形成新的肌肉，这代表了人类疾病杜氏肌营养不良症中坏死和炎症的关键特征。我们对切除的自体移植进行3D-OCT成像，并将OCT图像与配准的组织学图像进行比较。OCT图像能够很容易地将移植的坏死和炎症组织与下方宿主组织中完整的健康肌纤维区分开来。这些初步研究结果表明，随着进一步发展，3D-OCT可作为评估小动物肌肉形态和病理学的工具，特别是用于分析肌营养不良症的小鼠模型。
