Klyen Blake R, Scolaro Loretta, Shavlakadze Tea, Grounds Miranda D, Sampson David D
Optical + Biomedical Engineering Laboratory, School of Electrical, Electronic and Computer Engineering, The University of Western Australia, M018, 35 Stirling Highway, Crawley, Western Australia 6009, Australia.
Skeletal Muscle Research Group, School of Anatomy, Physiology and Human Biology, The University of Western Australia, M309, 35 Stirling Highway, Crawley, Western Australia 6009, Australia.
Biomed Opt Express. 2014 Mar 19;5(4):1217-32. doi: 10.1364/BOE.5.001217. eCollection 2014 Apr 1.
We present the assessment of ex vivo mouse muscle tissue by quantitative parametric imaging of the near-infrared attenuation coefficient µt using optical coherence tomography. The resulting values of the local total attenuation coefficient µt (mean ± standard error) from necrotic lesions in the dystrophic skeletal muscle tissue of mdx mice are higher (9.6 ± 0.3 mm(-1)) than regions from the same tissue containing only necrotic myofibers (7.0 ± 0.6 mm(-1)), and significantly higher than values from intact myofibers, whether from an adjacent region of the same sample (4.8 ± 0.3 mm(-1)) or from healthy tissue of the wild-type C57 mouse (3.9 ± 0.2 mm(-1)) used as a control. Our results suggest that the attenuation coefficient could be used as a quantitative means to identify necrotic lesions and assess skeletal muscle tissue in mouse models of human Duchenne muscular dystrophy.
我们展示了使用光学相干断层扫描技术通过对近红外衰减系数µt进行定量参数成像来评估离体小鼠肌肉组织的方法。在mdx小鼠的营养不良性骨骼肌组织中，坏死病变部位的局部总衰减系数µt（平均值±标准误差）所得值（9.6±0.3 mm⁻¹）高于同一组织中仅含有坏死肌纤维的区域（7.0±0.6 mm⁻¹），并且显著高于完整肌纤维的值，无论是来自同一样本的相邻区域（4.8±0.3 mm⁻¹）还是用作对照的野生型C57小鼠的健康组织（3.9±0.2 mm⁻¹）。我们的结果表明，衰减系数可用作一种定量手段，以识别坏死病变并评估人类杜兴氏肌营养不良症小鼠模型中的骨骼肌组织。
