The University of Western Australia, School of Electrical, Electronic and Computer Engineering, Optical+Biomedical Engineering Laboratory, M018, 35 Stirling Highway, Crawley, Western Australia 6009, Australia.
J Biomed Opt. 2011 Jul;16(7):076013. doi: 10.1117/1.3598842.
Three-dimensional optical coherence tomography (3D-OCT) was used to image the structure and pathology of skeletal muscle tissue from the treadmill-exercised mdx mouse model of human Duchenne muscular dystrophy. Optical coherence tomography (OCT) images of excised muscle samples were compared with co-registered hematoxylin and eosin-stained and Evans blue dye fluorescence histology. We show, for the first time, structural 3D-OCT images of skeletal muscle dystropathology well correlated with co-located histology. OCT could identify morphological features of interest and necrotic lesions within the muscle tissue samples based on intrinsic optical contrast. These findings demonstrate the utility of 3D-OCT for the evaluation of small-animal skeletal muscle morphology and pathology, particularly for studies of mouse models of muscular dystrophy.
三维光学相干断层扫描（3D-OCT）用于对人类杜氏肌营养不良症 mdx 小鼠模型的骨骼肌组织的结构和病理学进行成像。对切除的肌肉样本的光学相干断层扫描（OCT）图像与共定位的苏木精和伊红染色以及 Evans 蓝染料荧光组织学进行了比较。我们首次展示了与共定位组织学高度相关的骨骼肌病理结构的 3D-OCT 图像。OCT 可以根据固有光学对比度识别肌肉组织样本中感兴趣的形态特征和坏死病变。这些发现证明了 3D-OCT 用于评估小型动物骨骼肌形态和病理学的效用，特别是用于研究肌肉营养不良的小鼠模型。
