Patwardhan Sachin V, Culver Joseph P
Washington University School of Medicine, Department of Radiology, Mallinckrodt Institute of Radiology, St. Louis, Missouri 63110, USA.
J Biomed Opt. 2008 Jan-Feb;13(1):011009. doi: 10.1117/1.2830656.
The quantitative accuracy of fluorescence and bioluminescence imaging of small animals can be improved by knowledge of the in situ optical properties of each animal. Obtaining in situ optical property maps is challenging, however, due to short propagation distances, requirements for high dynamic range, and the need for dense spatial, temporal, and spectral sampling. Using an ultrafast gated image intensifier and a pulsed laser source, we have developed a small animal diffuse optical tomography system with multiple synthetic modulation frequencies up to >1 GHz. We show that amplitude and phase measurements with useful contrast-to-noise ratios can be obtained for modulation frequencies over the range of approximately 250 to 1250 MHz. Experiments with tissue simulating phantoms demonstrate the feasibility of reconstructing the absorption and scattering optical properties in a small animal imaging system.
通过了解每只动物的原位光学特性，可以提高小动物荧光和生物发光成像的定量准确性。然而，由于传播距离短、对高动态范围的要求以及对密集空间、时间和光谱采样的需求，获取原位光学特性图具有挑战性。我们使用超快门控图像增强器和脉冲激光源，开发了一种具有高达>1 GHz的多个合成调制频率的小动物扩散光学层析成像系统。我们表明，对于大约250至1250 MHz范围内的调制频率，可以获得具有有用对比度噪声比的幅度和相位测量结果。使用组织模拟体模进行的实验证明了在小动物成像系统中重建吸收和散射光学特性的可行性。
