Sørensen Lars K, Havemose-Poulsen Anne, Sønder Søren U, Bendtzen Klaus, Holmstrup Palle
Department of Periodontology, School of Dentistry, Faculty of Health Sciences, University of Copenhagen, 20 Nørre Allé, Copenhagen, Denmark.
J Periodontol. 2008 Mar;79(3):477-85. doi: 10.1902/jop.2008.070309.
Microarray analysis of local and peripheral cells in subjects with immune-inflammatory diseases may identify candidate genes associated with these diseases. The present study identified differentially expressed genes in peripheral blood mononuclear cells (PBMCs) from subjects with untreated localized aggressive periodontitis (LAgP) or generalized aggressive periodontitis (GAgP). Differentially expressed genes were validated in groups of subjects with LAgP, GAgP, juvenile idiopathic arthritis (JIA), or rheumatoid arthritis (RA) and controls.
Candidate genes were identified by gene expression profiling of PBMCs using a microarray system in untreated gender-matched subjects with LAgP (N = 2) or GAgP (N = 3) and controls (N = 2) younger than 35 years of age. The microarray results were validated by real-time reverse transcription-polymerase chain reaction (RT-PCR) using PBMCs from 103 individuals, including groups of subjects with LAgP (N = 18), GAgP (N = 27), JIA (N = 10), or RA (N = 23) and controls (N = 25).
Of 53 differentially expressed candidate genes identified in subjects with LAgP, 14 were involved in immune responses and inflammatory processes. Of these, the RT-PCR validation confirmed that Toll-like receptor 2 gene (TLR2) and myomesin 2 gene had a significantly higher expression in subjects with LAgP than in controls. RT-PCR also showed increased expression of TLR2 in subjects with RA. Comparison of subjects with GAgP to controls using microarray analysis identified only three upregulated genes.
Several genes upregulated in subjects with LAgP were related to immune responses including TLR2 and myomesin 2.
对免疫炎症性疾病患者的局部和外周细胞进行微阵列分析，可能会鉴定出与这些疾病相关的候选基因。本研究鉴定了未经治疗的局限性侵袭性牙周炎（LAgP）或广泛性侵袭性牙周炎（GAgP）患者外周血单核细胞（PBMC）中差异表达的基因。在LAgP、GAgP、幼年特发性关节炎（JIA）、类风湿关节炎（RA）患者组和对照组中对差异表达基因进行了验证。
使用微阵列系统对年龄小于35岁的未经治疗的性别匹配的LAgP患者（N = 2）、GAgP患者（N = 3）和对照组（N = 2）的PBMC进行基因表达谱分析，以鉴定候选基因。使用来自103名个体的PBMC通过实时逆转录聚合酶链反应（RT-PCR）对微阵列结果进行验证，这些个体包括LAgP患者组（N = 18）、GAgP患者组（N = 27）、JIA患者组（N = 10）、RA患者组（N = 23）和对照组（N = 25）。
在LAgP患者中鉴定出的53个差异表达候选基因中，有14个参与免疫反应和炎症过程。其中，RT-PCR验证证实Toll样受体2基因（TLR2）和肌间蛋白2基因在LAgP患者中的表达明显高于对照组。RT-PCR还显示RA患者中TLR2的表达增加。使用微阵列分析比较GAgP患者与对照组，仅鉴定出三个上调基因。
LAgP患者中上调的几个基因与免疫反应相关，包括TLR2和肌间蛋白2。
