Song Bao, Zhu Jing-Yan, Liu Jie, Wang Zhe-Hai, Shi Yan, Lü Li-Yan, Zheng Yan
Center of Basic Research, Shandong Institute of Tumor Prevention and Therapy, Jinan 250117, Shandong Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2008 Feb;16(1):97-100.
This study was aimed to explore the relationship between the single nucleotide polymorphisms of XPD (G23591A, A35931C) and individual susceptibility to non-Hodgkin's lymphomas (NHL) in Shandong populations. XPD gene polymorphism in 309 cases of NHL and 305 healthy controls were detected using PCR-restriction fragment length polymorphism assay in a case-control molecular epidemiology study. The association between gene polymorphism and the risk of NHL were examined through comparing odds ratio (OR) and 95% confidence interval (CI) between two groups. The results showed that no significant association between the XPD (G23591A, A35931C) polymorphism and the risk of whole NHL was shown at first. In the further analysis, all NHL cases were divided into four groups: follicular lymphoma (FL) group, diffuse large B-cell lymphoma (DLBCL) group, T-cell lymphoma group and other B-cell lymphoma group. Frequencies of XPD 23591GA + AA genotypes were 16.3%, 18.0%, 10.5% and 18.4% in each subgroup respectively, while 12.5% in control. Individuals carrying GA + AA genotype had 1.43, 1.58, 0.89 and 1.50-fold risk of NHL sub groups as much as GG genotype, but no statistically significant difference between subgroups and control was found (p>0.05); frequencies of XPD 35931AC + CC genotypes were 15.2%, 15.8%, 18.4% and 12.5% in each subgroup, while 11.5% in control. Individuals carrying AC + CC genotype had 1.41, 1.48, 1.75 and 1.12-fold risk of NHL subgroup as much as AA genotype, but there were also no statistically significant difference between each subgroup and control (p>0.05). It is concluded that the gene polymorphism of XPD (G23591A, G935931C) does not associate with the risk of developing NHL in Shandong populations.
本研究旨在探讨山东人群中XPD基因单核苷酸多态性（G23591A、A35931C）与非霍奇金淋巴瘤（NHL）个体易感性之间的关系。在一项病例对照分子流行病学研究中，采用聚合酶链反应-限制性片段长度多态性分析方法检测了309例NHL患者和305例健康对照者的XPD基因多态性。通过比较两组的比值比（OR）和95%置信区间（CI），研究基因多态性与NHL发病风险之间的关联。结果显示，起初XPD（G23591A、A35931C）多态性与整体NHL发病风险之间未显示出显著关联。在进一步分析中，将所有NHL病例分为四组：滤泡性淋巴瘤（FL）组、弥漫性大B细胞淋巴瘤（DLBCL）组、T细胞淋巴瘤组和其他B细胞淋巴瘤组。XPD 23591GA + AA基因型在各亚组中的频率分别为16.3%、18.0%、10.5%和18.4%，而在对照组中为12.5%。携带GA + AA基因型的个体患NHL各亚组的风险是GG基因型个体的1.43、1.58、0.89和1.50倍，但各亚组与对照组之间差异无统计学意义（p>0.05）；XPD 35931AC + CC基因型在各亚组中的频率分别为15.2%、15.8%、18.4%和12.5%，而在对照组中为11.5%。携带AC + CC基因型的个体患NHL亚组的风险是AA基因型个体的1.41、1.48、1.75和1.12倍，但各亚组与对照组之间差异也无统计学意义（p>0.05）。结论是，XPD（G23591A、G935931C）基因多态性与山东人群患NHL的风险无关。
