Retinoic acid inhibits basement membrane protein biosynthesis while stimulating dome formation by Madin Darby canine kidney cells in hormonally defined serum-free medium.

The effect of retinoic acid on basement membrane protein biosynthesis and dome formation by Madin Darby canine kidney cells was examined. Retinoic acid inhibited the biosynthesis of laminin, collagen IV, and heparan sulfate proteoglycan by confluent MDCK monolayers in a hormonally defined serum-free medium. Retinoic acid inhibited laminin biosynthesis by as much as 70% after an 8 day incubation period. The inhibitory effect of retinoic acid on laminin biosynthesis preceded temporally the stimulatory effect of retinoic acid on dome formation. This observation is consistent with the existence of a causal relationship between these two phenomena. Not only did retinoic acid inhibit the biosynthesis of laminin, but in addition the biosynthesis of the cellular 67 kd laminin binding protein was inhibited.