用N，N-二甲基亚硝胺或甲磺酸甲酯进行体内处理对再生大鼠肝脏细胞质DNA聚合酶的影响。
Effect of treatment in vivo with N,N-dimethylnitrosamine or methyl methanesulphonate on the cytoplasmic DNA polymerase of regenerating rat liver.
作者信息
Salisbury J G, O'Connor P J
出版信息
Nucleic Acids Res. 1976 Jun;3(6):1561-8. doi: 10.1093/nar/3.6.1561.
Abstract
A 10-16 fold increase in rat liver cytoplasmic DNA polymerase (DNA polymerase-alpha) was observed by 24 hrs after two thirds partial hepatectomy. Injection of either N,N-dimethylnitrosamine (DMN) or methyl methanesulphonate (MMS) At 6 or 12 hrs after partial hepatectomy completely inhibited this increased production of polymerase, but when given at 20 hours they had less effect. Neither compound altered the molecular size distribution of the enzyme. These data indicate that the lowered levels of DNA polymerase-alpha could play a major role in the reduction in DNA synthesis which occurs after carcinogen treatment.
摘要
在三分之二部分肝切除术后24小时，观察到大鼠肝脏细胞质DNA聚合酶（DNA聚合酶α）增加了10 - 16倍。在部分肝切除术后6或12小时注射N，N - 二甲基亚硝胺（DMN）或甲磺酸甲酯（MMS）可完全抑制这种聚合酶产量的增加，但在20小时给药时，它们的作用较小。两种化合物均未改变该酶的分子大小分布。这些数据表明，DNA聚合酶α水平的降低可能在致癌物处理后发生的DNA合成减少中起主要作用。
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1
Effect of treatment in vivo with N,N-dimethylnitrosamine or methyl methanesulphonate on the cytoplasmic DNA polymerase of regenerating rat liver.用N，N-二甲基亚硝胺或甲磺酸甲酯进行体内处理对再生大鼠肝脏细胞质DNA聚合酶的影响。
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Effect of a single treatment with the alkylating carcinogens dimethylnitrosamine and methyl methanesulphonate on liver regenerating after partial hepatectomy. IV. Effect on methylase-mediated methylation of DNA.用烷化致癌物二甲基亚硝胺和甲磺酸甲酯单次处理对部分肝切除术后肝脏再生的影响。IV. 对DNA甲基化酶介导的DNA甲基化的影响。
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Repair replication of DNA in the intact animal following treatment with dimethylnitrosamine and with methyl methanesulphonate, studied by fractionation of nuclei in a zonal centrifuge.用二甲基亚硝胺和甲磺酸甲酯处理完整动物后，通过区带离心法对细胞核进行分级分离，研究DNA的修复复制情况。
Biochim Biophys Acta. 1976 Sep 20;447(1):53-64. doi: 10.1016/0005-2787(76)90095-2.
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