一种源自人脂皮质素5的新型抗炎肽。
A novel anti-inflammatory peptide from human lipocortin 5.
作者信息
Perretti M, Becherucci C, Mugridge K G, Solito E, Silvestri S, Parente L
机构信息
Sclavo Research Centre, Laboratory of Pharmacology, Siena, Italy.
出版信息
Br J Pharmacol. 1991 Jun;103(2):1327-32. doi: 10.1111/j.1476-5381.1991.tb09788.x.
Abstract
- A novel anti-inflammatory peptide (residues 204-212) of human recombinant lipocortin 5 (hrLC5) found on the high similarity region with uteroglobin is described. 2. Peptide 204-212 dose-dependently inhibited the contractions of rat isolated stomach strips elicited by porcine pancreatic phospholipase A2 (PLA2). Contractions caused by arachidonic acid (AA), prostaglandin E2 (PGE2) and 5-hydroxytryptamine were not affected. No direct enzyme inhibition was observed in a radiochemical assay. 3. PGE2 release by both human fibroblasts and rat macrophages was reduced by peptide 204-212 in a dose-dependent manner. 4. The development of carrageenin-induced oedema in rats was significantly inhibited by the local administration of peptide 204-212. 5. The pattern and potency of the biological effects of peptide 204-212 are similar to those of antiflammin 2, a lipocortin 1-derived peptide. 6. It is suggested that peptide 204-212 may represent the active site responsible for the anti-inflammatory properties of lipocortin 5.
摘要
- 描述了一种在人重组脂皮质素5（hrLC5）上与子宫珠蛋白高度相似区域发现的新型抗炎肽（第204 - 212位氨基酸残基）。2. 肽204 - 212剂量依赖性地抑制猪胰腺磷脂酶A2（PLA2）引起的大鼠离体胃条收缩。花生四烯酸（AA）、前列腺素E2（PGE2）和5 - 羟色胺引起的收缩不受影响。在放射化学分析中未观察到直接的酶抑制作用。3. 肽204 - 212以剂量依赖性方式降低人成纤维细胞和大鼠巨噬细胞释放PGE2。4. 局部给予肽204 - 212可显著抑制角叉菜胶诱导的大鼠水肿。5. 肽204 - 212的生物学效应模式和效力与脂皮质素1衍生肽抗炎症肽2相似。6. 提示肽204 - 212可能代表脂皮质素5抗炎特性的活性位点。
相似文献
1
A novel anti-inflammatory peptide from human lipocortin 5.一种源自人脂皮质素5的新型抗炎肽。
Br J Pharmacol. 1991 Jun;103(2):1327-32. doi: 10.1111/j.1476-5381.1991.tb09788.x.
2
'Antiflammins': two nonapeptide fragments of uteroglobin and lipocortin I have no phospholipase A2-inhibitory and anti-inflammatory activity.“抗炎症蛋白”：子宫珠蛋白和脂皮质蛋白I的两个九肽片段不具有磷脂酶A2抑制和抗炎活性。
FEBS Lett. 1990 May 21;264(2):171-5. doi: 10.1016/0014-5793(90)80241-a.
3
Studies on the anti-phospholipase A2 and anti-inflammatory activities of a synthetic nonapeptide from uteroglobin.子宫珠蛋白合成九肽的抗磷脂酶A2及抗炎活性研究
Arzneimittelforschung. 1991 Mar;41(3):240-3.
4
Anti-inflammatory actions of an N-terminal peptide from human lipocortin 1.人脂皮质素1 N端肽的抗炎作用
Br J Pharmacol. 1993 Mar;108(3):573-4. doi: 10.1111/j.1476-5381.1993.tb12843.x.
5
Studies on the anti-phospholipase A2 and anti-inflammatory activities of a uteroglobin fragment and related peptides.子宫珠蛋白片段及相关肽的抗磷脂酶A2和抗炎活性研究。
Arzneimittelforschung. 1993 Sep;43(9):997-1000.
6
Human recombinant lipocortin 1 has acute local anti-inflammatory properties in the rat paw edema test.人重组脂皮质蛋白1在大鼠足爪水肿试验中具有急性局部抗炎特性。
Proc Natl Acad Sci U S A. 1989 May;86(9):3428-32. doi: 10.1073/pnas.86.9.3428.
7
Antiflammins. Anti-inflammatory activity and effect on human phospholipase A2.抗炎症蛋白。抗炎活性及其对人磷脂酶A2的作用。
Biochem Pharmacol. 1992 Aug 4;44(3):519-25. doi: 10.1016/0006-2952(92)90444-n.
8
In vitro and in vivo effects of the anti-inflammatory peptides, antiflammins.
Biochem Pharmacol. 1992 Oct 6;44(7):1437-41. doi: 10.1016/0006-2952(92)90546-u.
9
Inhibition of phospholipase A2 by uteroglobin and antiflammin peptides.子宫珠蛋白和抗炎症肽对磷脂酶A2的抑制作用。
Adv Exp Med Biol. 1990;279:137-60. doi: 10.1007/978-1-4613-0651-1_9.
10
Novel anti-inflammatory peptides from the region of highest similarity between uteroglobin and lipocortin I.来自子宫珠蛋白和脂皮质蛋白I之间相似度最高区域的新型抗炎肽。
Nature. 1988 Oct 20;335(6192):726-30. doi: 10.1038/335726a0.
引用本文的文献
1
Conceivable difference in the anti-inflammatory mechanisms of lipocortins 1 and 5.脂皮质素 1 和 5 的抗炎机制可能存在差异。
Mediators Inflamm. 1993;2(2):109-13. doi: 10.1155/S0962935193000158.
2
Inhibition of smooth muscle contraction and platelet aggregation by peptide 204-212 of lipocortin 5: an attempt to define some structure requirements.脂皮质素 5 的肽 204-212 抑制平滑肌收缩和血小板聚集：尝试定义一些结构要求。
Mediators Inflamm. 1993;2(2):103-7. doi: 10.1155/S0962935193000146.
3
Antiflammin-2 activates the human formyl-peptide receptor like 1.抗炎症因子-2激活人源类甲酰肽受体1。
ScientificWorldJournal. 2006 Oct 25;6:1375-84. doi: 10.1100/tsw.2006.247.
4
Novel transglutaminase inhibitors reverse the inflammation of allergic conjunctivitis.新型转谷氨酰胺酶抑制剂可逆转过敏性结膜炎的炎症。
J Clin Invest. 2003 Jan;111(1):121-8. doi: 10.1172/JCI15937.
5
Resonance assignment and secondary structure determination and stability of the recombinant human uteroglobin with heteronuclear multidimensional NMR.利用异核多维核磁共振技术对重组人子宫珠蛋白进行共振归属、二级结构测定及稳定性研究。
J Biomol NMR. 1997 Jan;9(1):35-46. doi: 10.1023/a:1018619501038.
6
Tissue-specific expression of the gene coding for human Clara cell 10-kD protein, a phospholipase A2-inhibitory protein.编码人克拉拉细胞10-kD蛋白（一种磷脂酶A2抑制蛋白）的基因的组织特异性表达。
J Clin Invest. 1993 Nov;92(5):2099-109. doi: 10.1172/JCI116810.
7
Uteroglobin and uteroglobin-like proteins: the uteroglobin family of proteins.子宫珠蛋白及类子宫珠蛋白：子宫珠蛋白家族蛋白
J Endocrinol Invest. 1994 Sep;17(8):679-92. doi: 10.1007/BF03349687.
8
Annexins: calcium-binding proteins of multi-functional importance?膜联蛋白：具有多功能重要性的钙结合蛋白？
Med Microbiol Immunol. 1991;180(3):109-26. doi: 10.1007/BF00206115.
本文引用的文献
1
A sensitive method for the assay of 5-hydroxytryptamine.一种测定5-羟色胺的灵敏方法。
Br J Pharmacol Chemother. 1957 Sep;12(3):344-9. doi: 10.1111/j.1476-5381.1957.tb00146.x.
2
The selective release of phospholipase A2 by resident mouse peritoneal macrophages.驻留小鼠腹膜巨噬细胞对磷脂酶A2的选择性释放。
Biochem J. 1981 Nov 15;200(2):441-4. doi: 10.1042/bj2000441.
3
A comprehensive set of sequence analysis programs for the VAX.一套适用于VAX的综合序列分析程序。
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95. doi: 10.1093/nar/12.1part1.387.
4
Studies on the mediators of the acute inflammatory response induced in rats in different sites by carrageenan and turpentine.对角叉菜胶和松节油在大鼠不同部位诱导的急性炎症反应介质的研究。
J Pathol. 1971 May;104(1):15-29. doi: 10.1002/path.1711040103.
5
Cell biology. Consensus in exocytosis.细胞生物学。胞吐作用的共识。
Nature. 1986;320(6063):573. doi: 10.1038/320573a0.
6
Uteroglobin inhibits phospholipase A2 activity.子宫珠蛋白抑制磷脂酶A2的活性。
Life Sci. 1986 May 19;38(20):1813-9. doi: 10.1016/0024-3205(86)90135-9.
7
8
A comparison of the acute inflammatory response in adrenalectomised and sham-operated rats.肾上腺切除大鼠与假手术大鼠急性炎症反应的比较。
Br J Pharmacol. 1986 Jan;87(1):57-62. doi: 10.1111/j.1476-5381.1986.tb10156.x.
9
Eleventh Gaddum memorial lecture. Lipocortin and the mechanism of action of the glucocorticoids.第十一届加德姆纪念讲座。脂皮质素与糖皮质激素的作用机制
Br J Pharmacol. 1988 Aug;94(4):987-1015. doi: 10.1111/j.1476-5381.1988.tb11614.x.
Zotero 插件