Jansen K U, Shields J, Gordon J, Cairns J, Graber P, Bonnefoy J Y
Glaxo Institute for Molecular Biology S.A., Geneva, Switzerland.
J Recept Res. 1991;11(1-4):507-20. doi: 10.3109/10799899109066424.
Human CD23 (low affinity receptor for IgE) has been expressed in insect cells (Sf9) using the baculovirus expression system and the baculovirus transfer vector pAc373. Insect cells infected with a recombinant baculovirus coding for CD23 synthesized a polypeptide not found in wild-type infected insect cells that had antigenic properties similar to natural CD23 produced in RPMI 8866 cells. Surface expression of recombinant CD23 was demonstrated by its ability to bind IgE. Recombinant CD23 expressed in insect cells had a slightly lower molecular weight (43 kDa) than that of natural CD23 (45 kDa) from RPMI 8866 cells as detected by SDS-PAGE followed by Western-blotting. Affinity-purified recombinant CD23 from infected insect cells showed B-cell growth promoting activitiy. These observations demonstrate for the first time that biologically active recombinant CD23 can be produced by the baculovirus expression system, thus providing a useful source of recombinant material to elucidate the biological functions of CD23.
人CD23（IgE低亲和力受体）已利用杆状病毒表达系统和杆状病毒转移载体pAc373在昆虫细胞（Sf9）中表达。用编码CD23的重组杆状病毒感染的昆虫细胞合成了一种在野生型感染昆虫细胞中未发现的多肽，其抗原特性与RPMI 8866细胞中产生的天然CD23相似。重组CD23的表面表达通过其结合IgE的能力得以证明。通过SDS-PAGE随后进行Western印迹检测，在昆虫细胞中表达的重组CD23的分子量（43 kDa）略低于来自RPMI 8866细胞的天然CD23（45 kDa）。从感染昆虫细胞中亲和纯化的重组CD23显示出促进B细胞生长的活性。这些观察首次证明杆状病毒表达系统可产生具有生物活性的重组CD23，从而为阐明CD23的生物学功能提供了有用的重组材料来源。
