Descamps Simon, Arzouk Hayat, Bacou Francis, Bernardi Henri, Fedon Yann, Gay Stéphanie, Reyne Yves, Rossano Bernadette, Levin Jonathan
INRA, UMR 866 Différenciation cellulaire et croissance, 2 place Viala, 34060 Montpellier, France.
Cell Tissue Res. 2008 May;332(2):299-306. doi: 10.1007/s00441-008-0574-z. Epub 2008 Mar 6.
Secreted Frizzled-related proteins (Sfrps) are extracellular regulators of Wnt signalling and play important roles in developmental and oncogenic processes. They are known to be upregulated in regenerating muscle and in myoblast cultures but their function is unknown. Here, we show that the addition of recombinant Sfrp1 or Sfrp2 to C2C12 cell line cultures or to primary cultures of satellite cells results in the inhibition of myotube formation with no significant effect on the cell cycle or apoptosis. Even though at confluence, treated and untreated cultures are identical in appearance, analyses have shown that, for maximum effect, the cells have to be treated while they are proliferating. Furthermore, removal of Sfrp from the culture medium during differentiation restores normal myotube formation. We conclude that Sfrp1 and Sfrp2 act to prevent myoblasts from entering the terminal differentiation process.
分泌型卷曲相关蛋白（Sfrps）是Wnt信号通路的细胞外调节因子，在发育和致癌过程中发挥重要作用。已知它们在再生肌肉和肌成纤维细胞培养物中上调，但其功能尚不清楚。在这里，我们表明，将重组Sfrp1或Sfrp2添加到C2C12细胞系培养物或卫星细胞原代培养物中会导致肌管形成受到抑制，而对细胞周期或细胞凋亡没有显著影响。尽管在汇合时，处理过的和未处理过的培养物外观相同，但分析表明，为了达到最大效果，细胞必须在增殖时进行处理。此外，在分化过程中从培养基中去除Sfrp可恢复正常的肌管形成。我们得出结论，Sfrp1和Sfrp2的作用是阻止成肌细胞进入终末分化过程。
