部分肝切除术后肝脏再生中肿瘤坏死因子-α转换酶的表达
Expression of tumor necrosis factor-alpha converting enzyme in liver regeneration after partial hepatectomy.
作者信息
Lin Xian-Ming, Liu Ying-Bin, Zhou Fan, Wu Yu-Lian, Chen Li, Fang He-Qing
机构信息
Department of Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, Zhejiang Province, China.
出版信息
World J Gastroenterol. 2008 Mar 7;14(9):1353-7. doi: 10.3748/wjg.14.1353.
AIM
To study the expression of tumor necrosis factor-alpha converting enzyme (TACE) and evaluate its significance in liver regeneration after partial hepatectomy in vivo.
METHODS
Male SD rats underwent 70% partial hepatectomy. The remaining liver and spleen tissue samples were collected at indicated time points after hepatectomy. TACE expression was investigated by Western blotting, immunohistochemistry, and serial section immunostaining.
RESULTS
Expression of TACE in liver and spleen tissues after partial hepatectomy was a time-dependent alteration, reaching a maximal level between 24 and 48 h and remaining elevated for more than 168 h. TACE protein was localized to mononuclear cells (MNC), which infiltrated the liver from the spleen after hepatectomy. The kinetics of TACE expression was in accordance with the number of TACE-staining MNCs and synchronized with those of transforming growth factor-alpha (TGFalpha). In addition, TACE-staining MNC partially overlapped with CD3+ T lymphocytes.
CONCLUSION
TACE may be involved in liver regeneration by pathway mediated with TGFalpha-EGFR in the cell-cycle progressive phase in vivo. TACE production and effect by paracrine may be a pathway of involvement in liver regeneration for the activated CD3+ T lymphocytes.
目的
研究肿瘤坏死因子-α转化酶（TACE）的表达，并评估其在体内部分肝切除术后肝再生中的意义。
方法
雄性SD大鼠接受70%部分肝切除术。在肝切除术后的指定时间点收集剩余的肝脏和脾脏组织样本。通过蛋白质印迹法、免疫组织化学和连续切片免疫染色研究TACE的表达。
结果
部分肝切除术后肝脏和脾脏组织中TACE的表达呈时间依赖性变化，在24至48小时之间达到最高水平，并持续升高超过168小时。TACE蛋白定位于单核细胞（MNC），肝切除术后这些细胞从脾脏浸润到肝脏。TACE表达的动力学与TACE染色的MNC数量一致，并与转化生长因子-α（TGFα）的动力学同步。此外，TACE染色的MNC与CD3+ T淋巴细胞部分重叠。
结论
在体内细胞周期进展阶段，TACE可能通过TGFα-表皮生长因子受体（EGFR）介导的途径参与肝再生。旁分泌产生的TACE及其作用可能是活化的CD3+ T淋巴细胞参与肝再生的一条途径。
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