Hung T-H, Chen S-F, Hsu J-J, Hsieh C-C, Hsueh S, Hsieh T-T
Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, 199 Dun-hua North Road, Taipei 105, Taiwan.
Placenta. 2006 Sep-Oct;27(9-10):996-1006. doi: 10.1016/j.placenta.2005.11.002. Epub 2005 Dec 27.
Chorioamnionitis increases the risk of preterm labour and is associated with adverse neonatal outcomes including cerebral palsy. Tumour necrosis factor-alpha (TNF-alpha) derived from the gestational tissues (placenta, fetal membranes and maternal decidua) is thought to play a pivotal role in the induction of cytokine response in chorioamnionitis. Tumour necrosis factor-alpha converting enzyme (TACE) is essential for the release of TNF-alpha. Our aim was to determine whether the expression of TACE is increased in human gestational tissues from pregnancies complicated by chorioamnionitis, and whether lipopolysaccharide (LPS) causes increased expression of TACE in the human gestational tissues in vitro. The immunostaining of TACE was generally more intense, in particular in the syncytiotrophoblast and stromal cells, in villous samples from pregnancies complicated by chorioamnionitis than those from normal pregnancies. Increased immunoreactivity of TACE was also noted in the amnion and choriodecidua. In parallel, there was an increased infiltration of monocytes/macrophages within the villous stroma and choriodecidua. As a complement to our in vivo findings, LPS significantly increased the levels of mRNA and protein of TACE in a dose-dependent response in villous and fetal membrane explant cultures. Together, our results imply a potential role of TACE in the pathogenesis of chorioamnionitis.
绒毛膜羊膜炎会增加早产风险，并与包括脑瘫在内的不良新生儿结局相关。源自妊娠组织（胎盘、胎膜和母体蜕膜）的肿瘤坏死因子-α（TNF-α）被认为在绒毛膜羊膜炎的细胞因子反应诱导中起关键作用。肿瘤坏死因子-α转换酶（TACE）对TNF-α的释放至关重要。我们的目的是确定在合并绒毛膜羊膜炎的妊娠的人类妊娠组织中TACE的表达是否增加，以及脂多糖（LPS）在体外是否会导致人类妊娠组织中TACE表达增加。与正常妊娠的绒毛样本相比，合并绒毛膜羊膜炎的妊娠的绒毛样本中TACE的免疫染色通常更强，尤其是在合体滋养层细胞和基质细胞中。在羊膜和绒毛膜蜕膜中也观察到TACE的免疫反应性增加。同时，绒毛基质和绒毛膜蜕膜内单核细胞/巨噬细胞的浸润增加。作为我们体内研究结果的补充，LPS在绒毛和胎膜外植体培养物中以剂量依赖性反应显著增加了TACE的mRNA和蛋白质水平。总之，我们的结果表明TACE在绒毛膜羊膜炎的发病机制中具有潜在作用。
