Mertens K, Rogiers V, Sonck W, Vercruysse A
Department of Toxicology, Vrije Universiteit, Brussels, Belgium.
Cell Biol Toxicol. 1991 Apr;7(2):101-10. doi: 10.1007/BF00122825.
Reduced and oxidized glutathione contents of adult rat hepatocytes in pure culture and in co-culture with rat epithelial cells were measured under various medium conditions. To the standard medium fetal calf serum, nicotinamide, H2SeO3, dimethylsulphoxide or no supplements were added. For freshly isolated hepatocytes, intracellular contents of 24 +/- 7 nmol reduced and 0.7 +/- 0.2 nmol oxidized glutathione/mg cellular protein were obtained, respectively. In pure culture as well as in co-culture and regardless of the medium conditions involved, the protein content stays constant during the culture time with the exception of a decrease in protein content after 6 days of pure culture, caused by deterioration and loss of the hepatocytes. In both culture systems, an initial increase in intracellular reduced glutathione levels was observed, followed by a decrease and a quick normalisation in co-culture. On the contrary, in pure culture, the decrease was slower, but not transient and a stabilized situation was never reached. The various supplementations of the culture media had no significant effect on the intracellular reduced glutathione contents of both culture systems. As far as the intra- and the extracellular oxidized glutathione contents and the extracellular reduced form are concerned, these were only present in small amounts.
在各种培养基条件下，测量了纯培养以及与大鼠上皮细胞共培养的成年大鼠肝细胞中还原型和氧化型谷胱甘肽的含量。向标准培养基中添加胎牛血清、烟酰胺、亚硒酸、二甲基亚砜或不添加任何补充剂。对于新鲜分离的肝细胞，细胞内还原型谷胱甘肽含量为24±7 nmol/mg细胞蛋白，氧化型谷胱甘肽含量为0.7±0.2 nmol/mg细胞蛋白。在纯培养和共培养中，无论涉及何种培养基条件，除了纯培养6天后由于肝细胞的退化和损失导致蛋白质含量下降外，培养期间蛋白质含量保持恒定。在两种培养系统中，均观察到细胞内还原型谷胱甘肽水平最初升高，随后下降，在共培养中迅速恢复正常。相反，在纯培养中，下降较慢，但不是短暂的，且从未达到稳定状态。培养基的各种补充对两种培养系统中细胞内还原型谷胱甘肽含量没有显著影响。就细胞内和细胞外氧化型谷胱甘肽含量以及细胞外还原型谷胱甘肽而言，它们的含量都很少。
