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合成白蛋白的人胎肝细胞的长期培养与传代。

Long-term culture and passage of human fetal liver cells that synthesize albumin.

作者信息

Sells M A, Chernoff J, Cerda A, Bowers C, Shafritz D A, Kase N, Christman J K, Acs G

出版信息

In Vitro Cell Dev Biol. 1985 Apr;21(4):216-20. doi: 10.1007/BF02620932.

DOI:10.1007/BF02620932
PMID:4008435
Abstract

Long-term cultures of hepatocytes were established from livers of human fetuses obtained by abortion at 18 to 23 wk of gestation. Cells obtained by collagenase dissociation of liver were maintained in defined serum-free medium on a substratum of positively charged plastic. Under these conditions, the cells divide and form a confluent monolayer. After multiple passages over a period of 3 mo., the cells retained an epithelioid morphology and continued to synthesize and secrete albumin.

摘要

从妊娠18至23周人工流产获取的人胎儿肝脏中建立了肝细胞长期培养体系。通过胶原酶消化肝脏获得的细胞在带正电荷的塑料基质上的限定无血清培养基中培养。在这些条件下，细胞分裂并形成汇合的单层。经过3个月的多次传代后，细胞保持上皮样形态，并继续合成和分泌白蛋白。

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本文引用的文献

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Hepatocyte proliferation in vitro: its dependence on the use of serum-free hormonally defined medium and substrata of extracellular matrix.体外肝细胞增殖：其对无血清激素限定培养基及细胞外基质底物使用的依赖性。
Proc Natl Acad Sci U S A. 1984 Mar;81(5):1411-5. doi: 10.1073/pnas.81.5.1411.
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Cell types in rat liver cultures: their identification and isolation.大鼠肝脏培养中的细胞类型：它们的鉴定与分离。
Mol Cell Biochem. 1983;53-54(1-2):23-33. doi: 10.1007/BF00225244.
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[Excretion of HBs and HBe antigens by normal human adult hepatocytes infected in vitro by hepatitis B virus].[体外感染乙肝病毒的正常成人肝细胞对乙肝表面抗原和乙肝e抗原的排泄]
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