兔L-古洛糖酸3-脱氢酶的结晶及初步X射线晶体学分析
Crystallization and preliminary X-ray crystallographic analysis of rabbit L-gulonate 3-dehydrogenase.
作者信息
Asada Yukuhiko, Kuroishi Chizu, Ukita Yoko, Sumii Rie, Endo Satoshi, Matsunaga Toshiyuki, Hara Akira, Kunishima Naoki
机构信息
Advanced Protein Crystallography Research Group, RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo-gun, Hyogo 679-5148, Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Mar 1;64(Pt 3):228-30. doi: 10.1107/S1744309108004326. Epub 2008 Feb 29.
Abstract
Rabbit L-gulonate 3-dehydrogenase was crystallized using the oil-microbatch method at 295 K. X-ray diffraction data were collected to 1.70 A resolution from a crystal at 100 K using synchrotron radiation. The crystal belongs to the C-centred monoclinic space group C2, with unit-cell parameters a = 71.81, b = 69.08, c = 65.64 A, beta = 102.7 degrees. Assuming the presence of a monomeric protomer in the asymmetric unit gives a V(M) value of 2.21 A(3) Da(-1) and a solvent content of 44.4%. A cocrystal with NADH, which was isomorphous to the apo form, was also prepared and diffraction data were collected to 1.85 A resolution using Cu Kalpha radiation at 100 K.
摘要
采用油微批量法在295 K下使兔L-古洛糖酸3-脱氢酶结晶。使用同步辐射在100 K下从晶体收集到分辨率为1.70 Å的X射线衍射数据。该晶体属于C心单斜空间群C2，晶胞参数a = 71.81、b = 69.08、c = 65.64 Å，β = 102.7°。假设不对称单元中存在单体原体，则V(M)值为2.21 ų Da⁻¹，溶剂含量为44.4%。还制备了与NADH的共晶体，其与脱辅基形式同晶型，并在100 K下使用Cu Kα辐射收集到分辨率为1.85 Å的衍射数据。
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