放射免疫分析法测定促肾上腺皮质激素促进猫离体肾上腺皮质细胞释放前列腺素E2和前列腺素F2α的情况。

Radioimmunoassay measurement of ACTH-facilitated PGE2 and PGF2alpha release from isolated cat adrenocortical cells.

作者信息

Laychock S G, Rubin R P

出版信息

Prostaglandins. 1976 Apr;11(4):753-67. doi: 10.1016/0090-6980(76)90075-7.

Abstract

Prostaglandin (PG) biosynthesis by trypsin-dispersed cat adrenocortical cells was studied by radioimmunoassay (RIA). Parallel assays of incubation media using PGF2alpha and PGF1alpha antisera established that PGF2alpha is the primary PGF released by feline cortical cells. Following the reduction of PGE to PGF with sodium borohydride (NaBH4) these same two antisera were also used to identify PGE2 as the primary PGE released. RIA using a PGE antiserum confirmed the presence of PGE in the incubation medium. Steroidogenic concentrations of ACTH (50-250muU) enhanced PGE and PGF release, and indomethacin suppressed the ACTH-facilitated release. These studies provide additional evidence for ACTH-induced PG synthesis by feline cortical cells, and support the hypothesis that PGs play some role in the steroidogenic action of ACTH.

摘要

通过放射免疫分析法（RIA）研究了胰蛋白酶分散的猫肾上腺皮质细胞中前列腺素（PG）的生物合成。使用前列腺素F2α（PGF2α）和前列腺素F1α（PGF1α）抗血清对孵育培养基进行平行分析，确定PGF2α是猫皮质细胞释放的主要PGF。在用硼氢化钠（NaBH4）将前列腺素E（PGE）还原为PGF后，同样使用这两种抗血清来鉴定PGE2是释放的主要PGE。使用PGE抗血清的RIA证实了孵育培养基中存在PGE。促肾上腺皮质激素（ACTH）的类固醇生成浓度（50 - 250μU）可增强PGE和PGF的释放，而吲哚美辛可抑制ACTH促进的释放。这些研究为ACTH诱导猫皮质细胞合成PG提供了更多证据，并支持PG在ACTH的类固醇生成作用中发挥某种作用的假说。