Brzeszczynska Joanna, Luciak Marek, Gwozdzinski Krzysztof
School of Life Sciences, Heriot-Watt University, Edinburgh, UK.
Free Radic Res. 2008 Jan;42(1):40-8. doi: 10.1080/10715760701787693.
The aim of this study was to investigate erythrocytes rheological behaviour, membrane dynamics and erythrocytes susceptibility to disintegration upon strong oxidative stress induced by dialysis or by external H(2)O(2) among patients with CRF. EPR spectrometry was used to investigate alterations in physical state of cellular components. Generated ROS production induced: (1) significant increase of membrane fluidity in CRF erythrocytes treated with H(2)O(2) (p<0.005) and at 60 min of haemodialysis (p<0.05), (2) significant decrease of cytoskeletal protein-protein interactions (p<0.005) and (3) cellular osmotic fragility (p<0.0005). H(2)O(2) exacerbated these changes. Erythrocytes from CRF patients have changed rheological behaviour and present higher susceptibility to disintegration. Erythrocytes membrane characteristics indicate that CRF patients possess younger and more flexible cells, which are more susceptible to oxidative stress. This may contribute to the shortened survival of young erythrocytes in CRF patients.
本研究旨在调查慢性肾衰竭（CRF）患者在透析或外部过氧化氢（H₂O₂）诱导的强烈氧化应激下红细胞的流变学行为、膜动力学以及红细胞对崩解的易感性。采用电子顺磁共振光谱法研究细胞成分物理状态的变化。所产生的活性氧（ROS）诱导：（1）用H₂O₂处理的CRF红细胞（p<0.005）以及血液透析60分钟时（p<0.05）膜流动性显著增加；（2）细胞骨架蛋白-蛋白相互作用显著降低（p<0.005）；（3）细胞渗透脆性增加（p<0.0005）。H₂O₂加剧了这些变化。CRF患者的红细胞流变学行为发生改变，对崩解的易感性更高。红细胞膜特征表明，CRF患者拥有更年轻、更具柔韧性的细胞，这些细胞更容易受到氧化应激的影响。这可能导致CRF患者年轻红细胞存活时间缩短。
