Department of Biophysics, University of Białystok, Świerkowa 20B, 15-950, Białystok, Poland.
Cell Mol Biol Lett. 2012 Sep;17(3):333-48. doi: 10.2478/s11658-012-0014-7. Epub 2012 Apr 10.
Erythrocytes are constantly exposed to ROS due to their function in the organism. High tension of oxygen, presence of hemoglobin iron and high concentration of polyunsaturated fatty acids in membrane make erythrocytes especially susceptible to oxidative stress. A comparison of the antioxidant activities of polyphenol-rich plant extracts containing hydrolysable tannins from sumac leaves (Rhus typhina L.) and condensed tannins from grape seeds (Vitis vinifera L.) showed that at the 5-50 μg/ml concentration range they reduced to the same extent hemolysis and glutathione, lipid and hemoglobin oxidation induced by erythrocyte treatment with 400 μM ONOO(-) or 1 mM HClO. However, extract (condensed tannins) from grape seeds in comparison with extract (hydrolysable tannins) from sumac leaves stabilized erythrocytes in hypotonic NaCl solutions weakly. Our data indicate that both hydrolysable and condensed tannins significantly decrease the fluidity of the surface of erythrocyte membranes but the effect of hydrolysable ones was more profound. In conclusion, our results indicate that extracts from sumac leaves (hydrolysable tannins) and grape seeds (condensed tannins) are very effective protectors against oxidative damage in erythrocytes.
由于红细胞在机体中的功能，它们不断受到 ROS 的影响。氧的高张力、血红蛋白铁的存在以及膜中多不饱和脂肪酸的高浓度使红细胞特别容易受到氧化应激的影响。对富含多酚的植物提取物的抗氧化活性进行比较，这些提取物含有来自漆树叶（Rhus typhina L.）的可水解单宁和来自葡萄种子（Vitis vinifera L.）的缩合单宁。结果表明，在 5-50μg/ml 的浓度范围内，它们在相同程度上减少了由 400μM ONOO(-)或 1mM HClO 处理红细胞引起的溶血和谷胱甘肽、脂质和血红蛋白氧化。然而，与漆树叶提取物（可水解单宁）相比，葡萄种子提取物（缩合单宁）在低渗 NaCl 溶液中对红细胞的稳定性较弱。我们的数据表明，可水解单宁和缩合单宁都能显著降低红细胞膜表面的流动性，但可水解单宁的效果更为显著。总之，我们的结果表明，来自漆树叶（可水解单宁）和葡萄种子（缩合单宁）的提取物是红细胞氧化损伤的非常有效的保护剂。
