Lee Richard S, Monigatti Flavio, Lutchman Mohini, Patterson Thomas, Budnik Bogdan, Steen Judith A J, Freeman Michael R, Steen Hanno
Department of Urology, Harvard Medical School, Urological Diseases Research Center, Children's Hospital Boston, Boston, MA 02115, USA.
Proteomics. 2008 Mar;8(5):1097-112. doi: 10.1002/pmic.200700701.
The rat kidney matures during the first 2 wk of life, suggesting that temporal variations in the urinary proteome may occur during this period. We describe the urine proteome during postnatal development in the rat and demonstrate specific proteomic changes corresponding to developmental milestones. Urine was collected from 30 rats at five postnatal (P) days of life (P1, P3, P7, P14, and >P30) by bladder aspiration. The proteome was assessed by nano-ESI-LC-MS/MS. For identification, we used stringent criteria to provide a 1% false positive rate at the peptide level. The proteins in common at each time interval decreased during postnatal maturation. When comparing all five developmental times, six proteins were ubiquitously present. We detected 14 proteins involved with cellular adhesion, structure, or proliferation and differentiation only during neonatal development. Additionally, 30 proteins were specific to adults, of which 13 originated from the prostate or seminal vesicle. This is the first MS characterization of the normal urinary proteome in early postnatal rodent development that demonstrates distinct differences correlating with different stages of tissue maturation. Further characterization of the normal urinary proteome may provide the basis for identification of urinary biomarkers of diseases of the urinary tract.
大鼠肾脏在出生后的前2周内成熟，这表明在此期间尿蛋白质组可能会出现时间变化。我们描述了大鼠出生后发育过程中的尿液蛋白质组，并证明了与发育里程碑相对应的特定蛋白质组变化。通过膀胱穿刺从30只大鼠出生后5个不同时间点（出生后第1天、第3天、第7天、第14天和大于30天）收集尿液。通过纳升电喷雾液相色谱-串联质谱法评估蛋白质组。为了进行鉴定，我们采用严格的标准以在肽水平提供1%的假阳性率。在出生后成熟过程中，每个时间间隔内共有的蛋白质数量减少。在比较所有五个发育阶段时，有六种蛋白质普遍存在。我们仅在新生儿发育期间检测到14种与细胞黏附、结构或增殖及分化有关的蛋白质。此外，有30种蛋白质是成年大鼠特有的，其中13种来自前列腺或精囊。这是首次对出生后早期啮齿动物发育过程中的正常尿液蛋白质组进行质谱表征，证明了与组织成熟不同阶段相关的明显差异。对正常尿液蛋白质组的进一步表征可能为识别泌尿系统疾病的尿液生物标志物提供基础。
