Vaezzadeh Ali R, Steen Hanno, Freeman Michael R, Lee Richard S
Department of Urology, Children's Hospital Boston, Harvard Medical School, Boston, Massachusetts, USA.
J Urol. 2009 Sep;182(3):835-43. doi: 10.1016/j.juro.2009.05.001. Epub 2009 Jul 17.
Proteomics is a rapidly growing new discipline that has the potential to increase and improve the understanding of protein function and interaction in the context of systems biology. As a translational science it has the potential to identify many new therapeutic targets as well as diagnostic and prognostic biomarkers of disease. Proteomics approaches consist of a combination of powerful technologies such as protein/peptide separation, identification and bioinformatic detection, and quantitation based on powerful computational data processing tools. We present an overview of current proteomics technologies, a review of proteomics applications in urology and a perspective on the future of proteomics in clinical medicine.
A literature search was performed on the basic concepts of proteomics and technologies commonly used in this field. Advantages, challenges and limitations of current proteomics approaches are discussed, and proteomics applications in the field of urology are presented.
The proteomics approaches to answer clinical questions have only recently been introduced. Many different technologies have been used in this field, which is moving from simple description to quantitative clinical applications.
Proteomics offers new approaches to the study of genitourinary tract diseases, and the potential to identify clinically relevant biomarkers and new therapeutic targets.
蛋白质组学是一门迅速发展的新学科，有潜力在系统生物学背景下增进和改善对蛋白质功能及相互作用的理解。作为一门转化科学，它有潜力识别许多新的治疗靶点以及疾病的诊断和预后生物标志物。蛋白质组学方法由多种强大技术组合而成，如蛋白质/肽分离、鉴定和生物信息学检测，以及基于强大计算数据处理工具的定量分析。我们概述了当前的蛋白质组学技术，回顾了蛋白质组学在泌尿外科的应用，并展望了蛋白质组学在临床医学中的未来。
对蛋白质组学的基本概念以及该领域常用技术进行了文献检索。讨论了当前蛋白质组学方法的优势、挑战和局限性，并介绍了蛋白质组学在泌尿外科领域的应用。
用于回答临床问题的蛋白质组学方法直到最近才被引入。该领域已使用了许多不同技术，正从简单描述向定量临床应用发展。
蛋白质组学为泌尿生殖道疾病的研究提供了新方法，并有潜力识别临床相关生物标志物和新的治疗靶点。
