Libbey Jane E, Kirkman Nikki J, Smith Matthew C P, Tanaka Tomoko, Wilcox Karen S, White H Steve, Fujinami Robert S
Department of Pathology, University of Utah, Salt Lake City, Utah 84132-2305, USA.
Epilepsia. 2008 Jun;49(6):1066-74. doi: 10.1111/j.1528-1167.2008.01535.x. Epub 2008 Mar 6.
We demonstrate the establishment and characterization of a novel virus infection-induced seizure model in C57BL/6 mice.
C57BL/6 mice were infected with Theiler's murine encephalomyelitis virus (TMEV) or mock infected. Mice were followed for seizures, weight change, body temperature, motor function (righting reflex, rotorod) and neurological manifestations (inflammation [perivascular cuffing], pyknotic neurons, transforming growth factor [TGF]-beta expression).
C57BL/6 mice are susceptible to seizures induced by TMEV infection. Approximately 50% of C57BL/6 mice develop transient afebrile seizures. Motor function and coordination are impaired in seized mice. Pyramidal neuron pyknosis and TGF-beta expression correlate with seizure activity in the hippocampus.
The characterization of this model will enable the investigation of viral and immune contributions in the central nervous system to the development of seizure disorders in humans.
我们展示了在C57BL/6小鼠中建立并表征一种新型病毒感染诱导的癫痫模型。
C57BL/6小鼠感染泰勒氏鼠脑脊髓炎病毒（TMEV）或进行假感染。对小鼠进行癫痫发作、体重变化、体温、运动功能（翻正反射、转棒试验）和神经学表现（炎症[血管周围套袖现象]、固缩神经元、转化生长因子[TGF]-β表达）的监测。
C57BL/6小鼠易受TMEV感染诱导的癫痫发作影响。约50%的C57BL/6小鼠出现短暂的无热惊厥。癫痫发作的小鼠运动功能和协调性受损。锥体神经元固缩和TGF-β表达与海马体中的癫痫活动相关。
该模型的表征将有助于研究中枢神经系统中病毒和免疫因素对人类癫痫疾病发展的影响。
