Xu Dong-Qing, Huang Ji, Guo Shu-Qiao, Yang Xia, Bao Yong-Mei, Tang Hai-Juan, Zhang Hong-Sheng
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, PR China.
FEBS Lett. 2008 Apr 2;582(7):1037-43. doi: 10.1016/j.febslet.2008.02.052. Epub 2008 Mar 4.
We previously identified a salt and drought stress-responsive TFIIIA-type zinc finger protein gene ZFP252 from rice. Here we report the functional analysis of ZFP252 using gain- and loss-of-function strategies. We found that overexpression of ZFP252 in rice increased the amount of free proline and soluble sugars, elevated the expression of stress defense genes and enhanced rice tolerance to salt and drought stresses, as compared with ZFP252 antisense and non-transgenic plants. Our findings suggest that ZFP252 plays an important role in rice response to salt and drought stresses and is useful in engineering crop plants with enhanced tolerance to salt and drought stresses.
我们之前从水稻中鉴定出一个盐和干旱胁迫响应型TFIIIA类锌指蛋白基因ZFP252。在此，我们报道利用功能获得和功能缺失策略对ZFP252进行的功能分析。我们发现，与ZFP252反义植株和非转基因植株相比，水稻中ZFP252的过表达增加了游离脯氨酸和可溶性糖的含量，提高了胁迫防御基因的表达，并增强了水稻对盐和干旱胁迫的耐受性。我们的研究结果表明，ZFP252在水稻对盐和干旱胁迫的响应中起重要作用，并且在培育耐盐和耐旱胁迫能力增强的作物植株方面具有应用价值。
