Xu Yong, Shi Yuqing, Zhang Weijie, Zhu Kaikai, Feng Liguo, Wang Jianwen
College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China.
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.
Plants (Basel). 2024 Dec 22;13(24):3580. doi: 10.3390/plants13243580.
is a representative aromatic species. Wild roses are known for their strong tolerance to highly salty environments, whereas cultivated varieties of roses exhibit lower salt stress tolerance, limiting their development and industrial expansion. Previous studies have shown that C2H2-type zinc finger proteins play a crucial role in plants' resistance to abiotic stresses. In this study, 102 C2H2-type zinc finger genes () were identified in via a comprehensive approach. These genes were categorized into three lineages, and their motif constitutions were grouped into four classes. were distributed across all seven rose chromosomes, with 15 paralogous gene pairs identified within synteny regions. Additionally, 43 showed differential expression across various tissues under salt stress, with being the only gene consistently repressed in all tissues. Subcellular localization analysis revealed that the RrC2H2-8 protein was localized in the nucleus. The heterologous expression of in significantly improved its growth under salt stress compared to the wild-type (WT) plants. Furthermore, the malondialdehyde content in the roots of transgenic was significantly lower than that in the WT, suggesting that enhanced salt tolerance by reducing cellular damage. This study provides a systematic understanding of the family and identifies as a regulator of salt tolerance, laying a foundation for future research on the mechanisms of salt stress regulation by .
是一种典型的芳香植物。野生玫瑰以其对高盐环境的强耐受性而闻名，而玫瑰栽培品种的耐盐胁迫能力较低，限制了它们的发展和产业扩张。先前的研究表明，C2H2型锌指蛋白在植物对非生物胁迫的抗性中起关键作用。在本研究中，通过综合方法在中鉴定出102个C2H2型锌指基因（）。这些基因被分为三个谱系，其基序组成被分为四类。分布在所有七条玫瑰染色体上，在共线性区域内鉴定出15对旁系同源基因对。此外，43个在盐胁迫下的不同组织中表现出差异表达，是所有组织中唯一持续受抑制的基因。亚细胞定位分析表明，RrC2H2-8蛋白定位于细胞核。与野生型（WT）植物相比，在中的异源表达显著改善了其在盐胁迫下的生长。此外，转基因根部的丙二醛含量显著低于野生型，表明通过减少细胞损伤增强了耐盐性。本研究提供了对家族的系统理解，并鉴定出作为耐盐性的调节因子，为未来研究调节盐胁迫的机制奠定了基础。
