Kammenga Jan E, Phillips Patrick C, De Bono Mario, Doroszuk Agnieszka
Laboratory of Nematology, Wageningen University, Wageningen, The Netherlands.
Trends Genet. 2008 Apr;24(4):178-85. doi: 10.1016/j.tig.2008.01.001. Epub 2008 Mar 5.
Induced mutants in the nematode Caenorhabditis elegans are used to study genetic pathways of processes ranging from aging to behavior. The effects of such mutations are usually analyzed in a single wildtype background: N2. However, studies in other species demonstrate that the phenotype(s) of induced mutations can vary widely depending on the genetic background. Moreover, induced mutations in one genetic background do not reveal the allelic effects that segregate in natural populations and contribute to phenotypic variation. Because other wildtype Caenorhabditis spp., including C. elegans, are now available, we review how current mapping resources and methodologies within and between species support the use of Caenorhabditis spp. for studying genetic variation, with a focus on pathways associated with human disease.
线虫秀丽隐杆线虫中的诱导突变体被用于研究从衰老到行为等各种过程的遗传途径。此类突变的影响通常在单一野生型背景：N2中进行分析。然而，对其他物种的研究表明，诱导突变的表型可能因遗传背景的不同而有很大差异。此外，在一种遗传背景下的诱导突变无法揭示在自然种群中分离并导致表型变异的等位基因效应。由于现在有包括秀丽隐杆线虫在内的其他野生型秀丽隐杆线虫物种可供使用，我们综述了当前物种内部和物种之间的定位资源及方法如何支持利用秀丽隐杆线虫物种来研究遗传变异，重点关注与人类疾病相关的途径。
