Sterken Mark G, Snoek L Basten, Bosman Kobus J, Daamen Jikke, Riksen Joost A G, Bakker Jaap, Pijlman Gorben P, Kammenga Jan E
Laboratory of Nematology, Wageningen University, Wageningen, The Netherlands ; Laboratory of Virology, Wageningen University, Wageningen, The Netherlands.
Laboratory of Nematology, Wageningen University, Wageningen, The Netherlands.
PLoS One. 2014 Feb 24;9(2):e89760. doi: 10.1371/journal.pone.0089760. eCollection 2014.
Orsay virus (OrV) is the first virus known to be able to complete a full infection cycle in the model nematode species Caenorhabditis elegans. OrV is transmitted horizontally and its infection is limited by antiviral RNA interference (RNAi). However, we have no insight into the kinetics of OrV replication in C. elegans. We developed an assay that infects worms in liquid, allowing precise monitoring of the infection. The assay revealed a dual role for the RNAi response in limiting Orsay virus infection in C. elegans. Firstly, it limits the progression of the initial infection at the step of recognition of dsRNA. Secondly, it provides an inherited protection against infection in the offspring. This establishes the heritable RNAi response as anti-viral mechanism during OrV infections in C. elegans. Our results further illustrate that the inheritance of the anti-viral response is important in controlling the infection in the canonical wild type Bristol N2. The OrV replication kinetics were established throughout the worm life-cycle, setting a standard for further quantitative assays with the OrV-C. elegans infection model.
奥尔赛病毒（OrV）是已知的第一种能够在模式线虫物种秀丽隐杆线虫中完成完整感染周期的病毒。OrV通过水平传播，其感染受到抗病毒RNA干扰（RNAi）的限制。然而，我们对OrV在秀丽隐杆线虫中的复制动力学一无所知。我们开发了一种在液体中感染线虫的检测方法，能够精确监测感染情况。该检测方法揭示了RNAi反应在限制秀丽隐杆线虫中奥尔赛病毒感染方面的双重作用。首先，它在双链RNA识别步骤限制了初始感染的进展。其次，它为后代提供了对感染的遗传保护。这确立了可遗传的RNAi反应作为秀丽隐杆线虫中OrV感染期间的抗病毒机制。我们的结果进一步表明，抗病毒反应的遗传在控制经典野生型布里斯托尔N2中的感染方面很重要。在整个线虫生命周期中确定了OrV复制动力学，为使用OrV-秀丽隐杆线虫感染模型进行进一步的定量检测设定了标准。
