Nde Chantal W, Fakhr Mohamed K, Doetkott Curt, Logue Catherine M
The Great Plains Institute of Food Safety, Department of Veterinary and Microbiological Sciences, 1523 Centennial Boulevard, 130A Van Es Hall, North Dakota State University, Fargo, North Dakota 58105, USA.
J Food Prot. 2008 Feb;71(2):386-91. doi: 10.4315/0362-028x-71.2.386.
This study was aimed at comparing the ability of conventional culture, the iQ-Check real-time PCR kit, and invA PCR to detect Salmonella in naturally contaminated premarket and retail turkey parts. Premarket (n = 120) turkey parts collected from a commercial turkey processing plant, and retail turkey parts (n = 138) were examined. Both PCR methods detected a significantly greater (P < 0.05) number of positive samples when compared with the conventional culture method for the premarket turkey parts. The indices of total agreement between the conventional culture method and the iQ-Check kit for the premarket and retail parts were 79.2% (95% CI: 70.8, 86) and 90.6% (95% CI: 84.4, 94.9), respectively. When the conventional culture method was compared with invA PCR for Salmonella detection in the premarket and retail parts, the indices of total agreement were 75.8% (95% CI: 67.2, 83.2) and 84.1% (95% CI: 76.9, 89.7), respectively. The rates of false positives (premarket: 31.9%, retail: 9.7%) and false negatives (premarket: 5.9%, retail: 9.7%) were determined between the culture method and the iQ-Check kit. When invA PCR was compared with the culture method, the rates of false positives (premarket: 37.7%, retail: 11.1%) and false negatives (premarket: 5.9%, retail: 18.3%) were obtained. The higher total agreement and the lower rates of both false positives and false negatives for the iQ-Check kit compared with invA PCR for both premarket and retail turkey parts corroborates the use of the iQ-Check kit as a screening tool for Salmonella in poultry meat.
本研究旨在比较传统培养法、iQ-Check实时荧光定量PCR试剂盒和invA基因PCR检测法在检测天然污染的上市前和零售火鸡产品中沙门氏菌的能力。对从一家商业火鸡加工厂采集的120份上市前火鸡产品以及138份零售火鸡产品进行了检测。与传统培养法相比，两种PCR方法检测出的上市前火鸡产品阳性样本数量显著更多（P < 0.05）。上市前和零售火鸡产品中，传统培养法与iQ-Check试剂盒的总体一致性指数分别为79.2%（95%置信区间：70.8, 86）和90.6%（95%置信区间：84.4, 94.9）。将传统培养法与invA基因PCR用于检测上市前和零售火鸡产品中的沙门氏菌时，总体一致性指数分别为75.8%（95%置信区间：67.2, 83.2）和84.1%（95%置信区间：76.9, 89.7）。确定了培养法与iQ-Check试剂盒之间的假阳性率（上市前：31.9%，零售：9.7%）和假阴性率（上市前：5.9%，零售：9.7%）。将invA基因PCR与培养法比较时，得出假阳性率（上市前：37.7%，零售：11.1%）和假阴性率（上市前：5.9%，零售：18.3%）。与invA基因PCR相比，iQ-Check试剂盒在上市前和零售火鸡产品中的总体一致性更高，假阳性率和假阴性率更低，这证实了iQ-Check试剂盒可作为禽肉中沙门氏菌的筛查工具。
