Glickman R M, Khorana J, Kilgore A
Science. 1976 Sep 24;193(4259):1254-5. doi: 10.1126/science.183265.
Indirect immunofluorescence techniques were employed to determine the distribution within intestinal epithelial cells of apolipoprotein B, a protein essential for the normal transport of fat. Isolated intestinal cells were prepared from rats either during active lipid absorption or after biliary diversion. Specific immunofluorescence from an antiserum to apolipoprotein B was detected in the apical portion of epithelial cells from bile-diverted animals, demonstrating that a pool of apolipoprotein B is present in the nonabsorptive epithelial cell and may be a component of intestinal cell membranes. During lipid absorption in normal rats, an early and sustained increase in immunofluorescence was demonstrated, consistent with an increase synthesis of apolipoprotein B during lipid absorption. This study demonstrates the presence of apolipoprotein B within intestinal epithelium and provides evidence for the participation of this apoprotein in intestinal lipid transport.
采用间接免疫荧光技术来确定载脂蛋白B在肠上皮细胞内的分布，载脂蛋白B是脂肪正常转运所必需的一种蛋白质。从处于活跃脂质吸收期的大鼠或胆汁分流后的大鼠中制备分离的肠细胞。在胆汁分流动物的上皮细胞顶端部分检测到了针对载脂蛋白B的抗血清的特异性免疫荧光，表明在非吸收性上皮细胞中存在载脂蛋白B池，并且它可能是肠细胞膜的一个组成部分。在正常大鼠的脂质吸收过程中，免疫荧光出现早期且持续的增加，这与脂质吸收过程中载脂蛋白B合成增加相一致。本研究证明了肠上皮内存在载脂蛋白B，并为这种载脂蛋白参与肠道脂质转运提供了证据。
