Speier Stephan, Nyqvist Daniel, Cabrera Over, Yu Jia, Molano R Damaris, Pileggi Antonello, Moede Tilo, Köhler Martin, Wilbertz Johannes, Leibiger Barbara, Ricordi Camillo, Leibiger Ingo B, Caicedo Alejandro, Berggren Per-Olof
The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital L1, SE-17176 Stockholm, Sweden.
Nat Med. 2008 May;14(5):574-8. doi: 10.1038/nm1701. Epub 2008 Mar 7.
Advanced imaging techniques have become a valuable tool in the study of complex biological processes at the cellular level in biomedical research. Here, we introduce a new technical platform for noninvasive in vivo fluorescence imaging of pancreatic islets using the anterior chamber of the eye as a natural body window. Islets transplanted into the mouse eye engrafted on the iris, became vascularized, retained cellular composition, responded to stimulation and reverted diabetes. Laser-scanning microscopy allowed repetitive in vivo imaging of islet vascularization, beta cell function and death at cellular resolution. Our results thus establish the basis for noninvasive in vivo investigations of complex cellular processes, like beta cell stimulus-response coupling, which can be performed longitudinally under both physiological and pathological conditions.
先进的成像技术已成为生物医学研究中在细胞水平研究复杂生物过程的宝贵工具。在此，我们介绍一种新的技术平台，该平台利用眼睛前房作为天然的体内窗口，对胰岛进行无创体内荧光成像。移植到小鼠眼中的胰岛在虹膜上植入，形成血管，保持细胞组成，对刺激作出反应并逆转糖尿病。激光扫描显微镜允许以细胞分辨率对胰岛血管生成、β细胞功能和死亡进行重复的体内成像。因此，我们的结果为复杂细胞过程的无创体内研究奠定了基础，例如β细胞刺激-反应偶联，这可以在生理和病理条件下纵向进行。
