将胰岛细胞移植到眼前房进行纵向体内成像。

Transplantation of Islets of Langerhans into the Anterior Chamber of the Eye for Longitudinal In Vivo Imaging.

机构信息

Paul Langerhans Institute Dresden (PLID) of the Helmholtz Zentrum München at the University Clinic Carl Gustav Carus of Technische Universität Dresden, Helmholtz Zentrum München, Neuherberg, Germany.

Institute of Physiology, Faculty of Medicine, Technische Universität Dresden, Dresden, Germany.

出版信息

Methods Mol Biol. 2020;2128:149-157. doi: 10.1007/978-1-0716-0385-7_11.

DOI:10.1007/978-1-0716-0385-7_11

PMID:32180192

Abstract

Noninvasive in vivo imaging techniques are attractive tools to longitudinally study various aspects of islet of Langerhans physiology and pathophysiology. Unfortunately, most imaging modalities currently applicable for clinical use do not allow the comprehensive investigation of islet cell biology due to limitations in resolution and/or sensitivity, while high-resolution imaging technologies like laser scanning microscopy (LSM) lack the penetration depth to assess islets of Langerhans within the pancreas. Significant progress in this area was made by the combination of LSM with the anterior chamber of the mouse eye platform, utilizing the cornea as a natural body window to study cell physiology of transplanted islets of Langerhans. We here describe the transplantation and longitudinal in vivo imaging of islets of Langerhans in the anterior chamber of the mouse eye as a versatile tool to study different features of islet physiology in health and disease.

摘要

非侵入性活体成像技术是一种很有吸引力的工具，可以用于纵向研究胰岛的各种生理学和病理生理学方面。不幸的是，由于分辨率和/或灵敏度的限制，目前大多数可用于临床的成像方式并不能全面研究胰岛细胞生物学，而像激光扫描显微镜（LSM）这样的高分辨率成像技术缺乏穿透深度来评估胰腺内的胰岛。通过将 LSM 与小鼠眼前房平台相结合，利用角膜作为天然的身体窗口，来研究移植胰岛的细胞生理学，在这一领域取得了重大进展。在这里，我们描述了将胰岛移植到小鼠眼前房，并进行纵向活体成像，作为一种研究健康和疾病状态下胰岛生理学不同特征的多功能工具。