Koşar Müberra, Göger Fatih, Can Başer K Hüsnü
Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskisehir, Turkey.
J Agric Food Chem. 2008 Apr 9;56(7):2369-74. doi: 10.1021/jf073516b. Epub 2008 Mar 8.
Antioxidant activities and phenolic compositions of the active fractions of Salvia virgata Jacq. (Lamiaceae) from Turkey were examined. The aerial part of S. virgata was extracted with different solvents in an order of increasing polarity such as hexane, ethyl acetate, methanol, and 50% aqueous methanol using a Soxhlet apparatus. Water extract was also prepared from S. virgata by reflux. All solvent fractions were investigated for their total phenolic contents, total flavonoids, flavonols, qualitative-quantitative compositions (by HPLC-PDA analysis), iron(III) reductive activities, free radical scavenging activities (using DPPH*), and effect upon linoleic acid peroxidation activities; also, the peroxidation level was determined by the TBA method. The results of activity tests given as IC50 values were estimated from nonlinear algorithm and compared with standards, viz., butylated hydroxytoluene, ascorbic acid, and gallic acid. Polar fractions were found to be more active for free radical activity whereas nonpolar fractions protected the peroxidation of linoleic acid. Rosmarinic acid was the most abundant component in the extracts, followed by caffeic acid and lutelin-7- O-glycoside.
对土耳其产鼠尾草（唇形科）活性部位的抗氧化活性和酚类成分进行了研究。使用索氏提取器，依次用极性递增的不同溶剂（如己烷、乙酸乙酯、甲醇和50%甲醇水溶液）对鼠尾草地上部分进行提取。还通过回流法制备了鼠尾草的水提取物。对所有溶剂提取物的总酚含量、总黄酮、黄酮醇、定性定量组成（通过高效液相色谱-光电二极管阵列检测分析）、铁（III）还原活性、自由基清除活性（使用二苯基苦味酰基自由基）以及对亚油酸过氧化活性的影响进行了研究；此外，通过硫代巴比妥酸法测定过氧化水平。以半数抑制浓度（IC50）值给出的活性测试结果通过非线性算法估算，并与标准物质（即丁基羟基甲苯、抗坏血酸和没食子酸）进行比较。发现极性提取物对自由基活性更具活性，而非极性提取物则能保护亚油酸的过氧化。迷迭香酸是提取物中含量最丰富的成分，其次是咖啡酸和木犀草素-7-O-糖苷。
