Department of Pharmaceutical Botany, Faculty of Pharmacy, Ankara University, Ankara 06560, Turkey.
Department of Pharmaceutical Botany, Faculty of Pharmacy, Erciyes University, Kayseri 38039, Turkey.
Molecules. 2022 Nov 26;27(23):8249. doi: 10.3390/molecules27238249.
subsp. , , and are traditionally used plants grown in Turkey. Methanol extracts were obtained from these plants and pharmacological activity studies and phytochemical analyses were carried out. To evaluate the phytochemical composition, spectrophotometric and chromatographic techniques were used. The extracts were evaluated for antioxidant activity by DPPH, ABTS radical scavenging, and FRAP assays. The cytotoxic effects of the extracts were investigated on DU145 prostate cancer and A549 lung cancer cell lines. The anti-inflammatory effects of extracts were investigated on the NO amount, TNF-α, IFN-γ, and PGE 2 levels in lipopolysaccharide-stimulated Raw 264.7 cells. The richest extract in terms of phenolic compounds (98.19 ± 1.64 mg/g) and total flavonoids (21.85 ± 0.64 mg/g) was identified as subsp. methanol extract. According to antioxidant activity determinations, the subsp. extract was found to be the most active extract. Finally, the subsp. methanol extract was revealed to be the most effective inhibitor of viability in the cytotoxic activity investigation, and the extract with the best anti-inflammatory action. The findings point to subsp. as a promising source of bioactive compounds in the transition from natural sources to industrial uses, such as new medications, cosmeceuticals, and nutraceuticals.
传统上，subsp. 、 和 被用作在土耳其种植的药用植物。从这些植物中提取甲醇提取物，并进行了药理学活性研究和植物化学分析。为了评估植物化学成分，使用了分光光度法和色谱技术。通过 DPPH、ABTS 自由基清除和 FRAP 测定法评估提取物的抗氧化活性。研究了提取物对 DU145 前列腺癌细胞系和 A549 肺癌细胞系的细胞毒性作用。研究了提取物对脂多糖刺激的 Raw 264.7 细胞中 NO 量、TNF-α、IFN-γ 和 PGE2 水平的抗炎作用。在酚类化合物（98.19 ± 1.64 mg/g）和总类黄酮（21.85 ± 0.64 mg/g）含量方面最丰富的提取物被鉴定为 subsp. 甲醇提取物。根据抗氧化活性测定，发现 subsp. 提取物是最活跃的提取物。最后，在细胞毒性活性研究中， subsp. 甲醇提取物被证明是对细胞活力最有效的抑制剂，并且是具有最佳抗炎作用的提取物。研究结果表明，subsp. 作为从天然来源向工业用途（如新药物、化妆品和营养保健品）过渡的有前途的生物活性化合物来源。
