Inoue Ryo, Tsuruta Takeshi, Nojima Iyori, Nakayama Keizo, Tsukahara Takamitsu, Yajima Takaji
Creative Research Initiative Sousei, Meiji Dairies Research Chair, Hokkaido University, Sapporo, Japan.
FEMS Immunol Med Microbiol. 2008 Apr;52(3):407-16. doi: 10.1111/j.1574-695X.2008.00390.x. Epub 2008 Mar 5.
Although there have been many fascinating studies on cryptdins, the information for each cryptdin isoform was not completely provided. In this study, the postnatal changes in the gene expression of cryptdin 1-6 were evaluated, and the patterns of change were compared between conventional and germ-free mice. Two patterns of postnatal change were observed: gene expression of cryptdins 1, 3 and 6 increased gradually, and that of cryptdins 2 and 5 increased rapidly. Gene expression of cryptdin 4 increased gradually in the ileum but rapidly in the jejunum. Conventional mice showed significantly higher gene expression for all isoforms than germ-free mice. Interestingly, the difference in the gene expression for cryptdin 2, 4 and 5 between the jejunum and ileum seemed to be increased by the presence of the luminal bacteria. The results indicate that cryptdin isoforms develop differently depending on the isoform type, and that the gene expression of all cryptdin isoforms was affected by the presence of the luminal bacteria.
尽管已经有许多关于隐窝蛋白的引人入胜的研究，但每种隐窝蛋白同工型的信息并未完全提供。在本研究中，评估了隐窝蛋白1 - 6基因表达的出生后变化，并比较了常规饲养小鼠和无菌小鼠之间的变化模式。观察到两种出生后变化模式：隐窝蛋白1、3和6的基因表达逐渐增加，而隐窝蛋白2和5的基因表达迅速增加。隐窝蛋白4在回肠中的基因表达逐渐增加，而在空肠中迅速增加。常规饲养小鼠的所有同工型基因表达均显著高于无菌小鼠。有趣的是，空肠和回肠之间隐窝蛋白2、4和5的基因表达差异似乎因肠腔细菌的存在而增大。结果表明，隐窝蛋白同工型根据同工型类型的不同而有不同的发育方式，并且所有隐窝蛋白同工型的基因表达均受肠腔细菌存在的影响。
