肠抗菌肽 HD5 的表达上调与 IgA 肾病的肾功能相关。
Upregulated Expression of Intestinal Antimicrobial Peptide HD5 Associated with Renal Function in IgA Nephropathy.
机构信息
Department of Nephrology, The First Affiliated Hospital Sun Yat-sen University, Guangzhou, Guangdong, China.
Key Laboratory of Nephrology, Guangdong Province, Guangzhou, Guangdong, China.
出版信息
Dis Markers. 2020 Feb 5;2020:2078279. doi: 10.1155/2020/2078279. eCollection 2020.
PURPOSE
It was reported that gut-kidney axis may play an important role in IgA nephropathy (IgAN). Previous five GWASs of different populations for IgAN have discovered several genes related to intestinal immunity, including gene. However, the roles of the encoded proteins of / which were called intestinal antimicrobial peptides HD5 and HD6 were not clear in kidney disease, such as IgAN. The purpose of this study was to clarify the association of HD5 and HD6 with IgAN.
METHODS
We measured HD5 and HD6 in serum, urine, and kidney of IgAN patients and normal controls by ELISA, Western blot, and immunofluorescence. The association of HD5 or HD6 levels with clinical and pathologic phenotypes was analyzed.
RESULTS
Serum levels of HD5 and HD6 were significantly higher in IgAN patients than those in normal controls. Baseline serum HD5 levels were significantly associated with eGFR ( = 0.002) and tubular atrophy/interstitial fibrosis ( = 0.002) and tubular atrophy/interstitial fibrosis ( = 0.002) and tubular atrophy/interstitial fibrosis ( = 0.002) and tubular atrophy/interstitial fibrosis (.
CONCLUSIONS
In IgAN patients, an elevated serum HD5 level at the time of renal biopsy was associated with poor renal outcomes. HD5 rather than HD6 was probably associated with renal function of IgAN patients.
目的
据报道，肠-肾轴可能在 IgA 肾病（IgAN）中发挥重要作用。先前针对不同人群的 5 项 IgAN 的全基因组关联研究（GWAS）发现了几个与肠道免疫相关的基因，包括 基因。然而，编码蛋白 / 被称为肠道抗菌肽 HD5 和 HD6 的作用在肾脏疾病如 IgAN 中尚不清楚。本研究旨在阐明 HD5 和 HD6 与 IgAN 的关系。
方法
我们通过 ELISA、Western blot 和免疫荧光法测量了 IgAN 患者和正常对照组血清、尿液和肾脏中的 HD5 和 HD6。分析了 HD5 或 HD6 水平与临床和病理表型的关系。
结果
IgAN 患者血清 HD5 和 HD6 水平明显高于正常对照组。基线时血清 HD5 水平与 eGFR（=0.002）和肾小管萎缩/间质纤维化（=0.002）显著相关，与 eGFR（=0.002）和肾小管萎缩/间质纤维化（=0.002）显著相关，与 eGFR（=0.002）和肾小管萎缩/间质纤维化（=0.002）显著相关，与 eGFR（=0.002）和肾小管萎缩/间质纤维化（=0.002）显著相关。
结论
在 IgAN 患者中，肾活检时血清 HD5 水平升高与肾脏不良结局相关。HD5 而不是 HD6 可能与 IgAN 患者的肾功能有关。
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