Department of Agricultural and Life Sciences, Kyoto Prefectural University, Hangi-cho, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan.
Laboratory of Nutritional Science for Animals, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.
Nutrients. 2018 Sep 3;10(9):1220. doi: 10.3390/nu10091220.
Luminal short-chain fatty acids (SCFA) are rapidly absorbed from the intestine and subsequently utilized by the host as substrate for metabolic energy production. In pigs, the energy contribution of SCFA is thought to be 30⁻76%. However, since absorption and blood flow dynamics of SCFA in pigs, particularly during the suckling⁻weaning period, remain unclear, we aimed to elucidate these phenomena. Thirty-two piglets were used in the present work. Cecal vein blood and digesta, and portal and abdominal vein blood were sampled from suckling (7-, 14-, 21- and 28-day-old) and weaned (weaning at 21 and 28 days of age) piglets. Four piglets from each group were euthanized. SCFA concentrations in blood samples were analyzed by a highly sensitive gas chromatography-mass spectrometry technique. Age at weaning tended to affect SCFA absorption. For example, acetate and propionate concentrations in the cecal vein tended to be higher in piglets weaned at day 21 than at day 28. SCFA concentrations in the abdominal vein tended to differ from those in other veins. Mucosal gene expression analysis suggested that monocarboxylate transporter 1 and occludin were associated in absorption of SCFA from the lumen into the blood of piglets.
腔体内短链脂肪酸 (SCFA) 可被快速从肠道吸收，并随后被宿主用作代谢能量产生的底物。在猪中，SCFA 的能量贡献被认为是 30⁻76%。然而，由于猪 SCFA 的吸收和血流动力学仍不清楚，尤其是在哺乳⁻断奶期间，因此我们旨在阐明这些现象。本研究使用了 32 头仔猪。从哺乳（7、14、21 和 28 日龄）和断奶（21 和 28 日龄断奶）仔猪采集盲肠静脉血和食糜，以及门静脉和腹静脉血。每组 4 头仔猪被安乐死。通过高灵敏度气相色谱-质谱技术分析血液样本中的 SCFA 浓度。断奶日龄会影响 SCFA 的吸收。例如，21 日龄断奶仔猪盲肠静脉中的乙酸盐和丙酸盐浓度往往高于 28 日龄断奶仔猪。腹静脉中的 SCFA 浓度往往与其他静脉中的 SCFA 浓度不同。黏膜基因表达分析表明，单羧酸转运蛋白 1 和紧密连接蛋白在仔猪腔体内 SCFA 从肠腔吸收到血液中起作用。
