镁对通透化艾氏腹水肿瘤细胞糖酵解的影响。

The effect of magnesium on glycolysis of permeabilized Ehrlich ascites tumor cells.

作者信息

Wolf F I, Bossi D, Cittadini A

机构信息

Institute of General Pathology, Catholic University, School of Medicine, Italy.

出版信息

Biochem Biophys Res Commun. 1991 Sep 16;179(2):1000-5. doi: 10.1016/0006-291x(91)91917-2.

DOI:10.1016/0006-291x(91)91917-2

PMID:1832860

Abstract

We have previously observed that extracellular Mg2+ influences the phosphofructokinase (PFK) activity of intact Ehrlich Ascites tumour cells (EATC). In this study we have investigated the mechanism by which Mg2+ modulates this key glycolytic enzyme in EATC made permeable to the cation by either digitonin or dextran sulphate. Results showed that when Mg2+ is freely permeable to the cytosol, the in vivo PFK activity, calculated as FDP/G6P ratio, is not increased as it is in intact cells. We also observed that in permeabilized cells Mg2+ determines the increase of glucose 6 phosphate (G6P), fructose 1,6 bisphosphate (FDP) and lactate production. We hypothesize that extracellular Mg2+ regulates PFK and glycolysis in these neoplastic cells not by entering the cytosol but by a specific interaction with the plasma membrane.

摘要

我们之前观察到细胞外镁离子（Mg2+）会影响完整的艾氏腹水瘤细胞（EATC）中磷酸果糖激酶（PFK）的活性。在本研究中，我们调查了Mg2+调节这种关键糖酵解酶的机制，该机制发生在通过洋地黄皂苷或硫酸葡聚糖使阳离子具有通透性的EATC中。结果显示，当Mg2+可自由通透进入细胞质时，以FDP/G6P比值计算的体内PFK活性并不像在完整细胞中那样增加。我们还观察到，在通透化细胞中，Mg2+会导致葡萄糖6磷酸（G6P）、果糖1,6二磷酸（FDP）和乳酸产量增加。我们推测，细胞外Mg2+并非通过进入细胞质，而是通过与质膜的特异性相互作用来调节这些肿瘤细胞中的PFK和糖酵解。