Schilling K, Börnig H
Acta Biol Med Ger. 1979;38(8):1101-14.
The effect of magnesium on the energy metabolism of Ehrlich ascites tumour cells was investigated using a method which allows to change the cellular content of magnesium rapidly at constant low calcium concentration. Cells, which have lost some of their magnesium, accumulate lactate slightly faster than non-treated cells. Mg-loading of these cells decreases the glycolytic flux rates to about 20% under aerobic and anaerobic conditions. The corresponding changes of some glycolytic metabolites suggest an inhibition of the HK-PFK-system. A similar inhibitory effect of Mg on O2-consumption in the absence and presence of glucose to about 20% and 15%, respectively, was observed. Despite the inhibition of the ATP-generating systems the ATP concentration increases under all conditions investigated, indicating an inhibition of ATP consuming systems. From experiments in the presence of ouabain, which inhibits the aerobic glycolysis to about 40% and 20%, in Mg-depleted and Mg-loaded cells, respectively, it is concluded that magnesium affects the active monovalent cation transport. Removal of magnesium increases the activity of the (Na+-K+)-ATPase and vice versa, presumably via changes of the cell membrane permeability.
采用一种能在恒定低钙浓度下快速改变细胞镁含量的方法，研究了镁对艾氏腹水癌细胞能量代谢的影响。失去部分镁的细胞比未处理的细胞积累乳酸的速度略快。这些细胞加载镁后，在有氧和无氧条件下，糖酵解通量速率降低至约20%。一些糖酵解代谢物的相应变化表明己糖激酶-磷酸果糖激酶系统受到抑制。在有无葡萄糖的情况下，镁对氧气消耗也有类似的抑制作用，分别约为20%和15%。尽管ATP生成系统受到抑制，但在所研究的所有条件下ATP浓度均升高，表明ATP消耗系统受到抑制。从哇巴因存在时的实验得出结论，哇巴因分别抑制缺镁和加载镁细胞中的有氧糖酵解约40%和20%，镁影响活性单价阳离子转运。去除镁会增加（Na+-K+）-ATP酶的活性，反之亦然，这可能是通过细胞膜通透性的变化实现的。
