Zygner Wojciech, Jaros Sławomir, Wedrychowicz Halina
Division of Parasitology and Parasitic Diseases, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw Agricultural University, Ciszewskiego 8, 02-786 Warsaw, Poland.
Vet Parasitol. 2008 May 6;153(1-2):139-42. doi: 10.1016/j.vetpar.2008.01.036. Epub 2008 Feb 3.
The purposes of this study were to specify the occurrence and prevalence of Babesia canis, Borrelia burgdorferi sensu lato, and Anaplasma phagocytophilum in ticks removed from dogs in Warsaw, and to determine the Borrelia species occurring in Ixodes ricinus ticks. Among 590 collected ticks, 209 were identified as I. ricinus, and 381 as Dermacentor reticulatus. DNA of B. canis was detected in 11% of D. reticulatus ticks. We found that 6.2% of I. ricinus ticks harbored B. burgdorferi s.l. specific DNA and 2.9% harbored A. phagocytophilum DNA. In these samples sequencing of the detected Borrelia amplicon confirmed infection with Borrelia afzelii genospecies. New sequences were submitted to the GenBank database (accession no. EU152128, EU152127, EU152126). This work is the first detection of B. afzelii and A. phagocytophilum in ticks from Warsaw, and the first survey for the prevalence of B. canis, B. afzelii, and A. phagocytophilum in ticks in central Poland.
本研究的目的是明确从华沙犬只身上采集的蜱中犬巴贝斯虫、伯氏疏螺旋体狭义种及嗜吞噬细胞无形体的发生情况和流行率，并确定蓖麻硬蜱中存在的伯氏疏螺旋体种类。在采集的590只蜱中，209只被鉴定为蓖麻硬蜱，381只为网纹革蜱。在11%的网纹革蜱中检测到犬巴贝斯虫的DNA。我们发现6.2%的蓖麻硬蜱携带伯氏疏螺旋体狭义种特异性DNA，2.9%携带嗜吞噬细胞无形体DNA。在这些样本中，对检测到的伯氏疏螺旋体扩增子进行测序证实感染了阿氏疏螺旋体基因种。新序列已提交至GenBank数据库（登录号EU152128、EU152127、EU152126）。这项工作首次在华沙的蜱中检测到阿氏疏螺旋体和嗜吞噬细胞无形体，也是首次对波兰中部蜱中犬巴贝斯虫、阿氏疏螺旋体和嗜吞噬细胞无形体的流行情况进行调查。
