De Caro Josiane, Eydoux Cécilia, Chérif Slim, Lebrun Régine, Gargouri Youssef, Carrière Frédéric, De Caro Alain
Laboratoire d'Enzymologie Interfaciale et de Physiologie de la Lipolyse, Unité Propre de Recherche 9025, France.
Comp Biochem Physiol B Biochem Mol Biol. 2008 May;150(1):1-9. doi: 10.1016/j.cbpb.2008.01.007. Epub 2008 Feb 7.
The occurrence of classical pancreatic lipase (PL) and pancreatic lipase-related proteins 1 and 2 (PLRP1s and 2) in the pancreas of ten mammalian species (humans, pig, rat, guinea pig, coypu, rabbit, horse, ox, goat and sheep) and two bird species (ostrich and turkey) was investigated. The lipases were purified from delipidated pancreas and identified based on the results of Western blotting analysis with anti-human PLRP2 serum, the catalytic properties and N-terminal microsequencing data. PLRP2s were detected in the pancreas of monogastric herbivorous animals (guinea pig, coypu, rabbit and horse) but not in that of ruminant herbivorous animals (ox, goat and sheep). The pancreas of carnivorous animals (dogs and cats) does not have any detectable PLRP2, but contains high levels of PL and PLRP1. By contrast, the pancreas of omnivorous animals (humans and rats) contains PL, PLRP1 and PLRP2, with the exception of porcine pancreas, where no PLRP2 was detected. In the case of bird (ostrich and turkey) pancreases, only classical PL was detected. The substrate specificity of PLRP2s was investigated using phospholipid micelles and synthetic monomolecular galactolipid films. Like human PLRP2, rabbit and horse PLRP2s are galactolipases. In polygastric herbivorous animals (ruminants), however, galactolipids are hydrolyzed via microbial enzymatic processes (involving galactolipases). The absence of galactolipids in carnivorous animals' diet may explain why no PLRP2s were detected here in the pancreas of these species.
研究了十种哺乳动物（人类、猪、大鼠、豚鼠、河狸鼠、兔子、马、牛、山羊和绵羊）以及两种鸟类（鸵鸟和火鸡）胰腺中经典胰脂肪酶（PL）和胰脂肪酶相关蛋白1和2（PLRP1和PLRP2）的存在情况。从脱脂胰腺中纯化脂肪酶，并根据抗人PLRP2血清的蛋白质免疫印迹分析结果、催化特性和N端微测序数据进行鉴定。在单胃草食动物（豚鼠、河狸鼠、兔子和马）的胰腺中检测到了PLRP2，而在反刍草食动物（牛、山羊和绵羊）的胰腺中未检测到。肉食动物（狗和猫）的胰腺中未检测到任何可检测到的PLRP2，但含有高水平的PL和PLRP1。相比之下，杂食动物（人类和大鼠）的胰腺含有PL、PLRP1和PLRP2，但猪胰腺除外，在猪胰腺中未检测到PLRP2。在鸟类（鸵鸟和火鸡）胰腺中，仅检测到经典的PL。使用磷脂微团和合成单分子半乳糖脂膜研究了PLRP2的底物特异性。与人类PLRP2一样，兔子和马的PLRP2是半乳糖脂酶。然而，在多胃草食动物（反刍动物）中，半乳糖脂通过微生物酶促过程（涉及半乳糖脂酶）被水解。肉食动物饮食中不存在半乳糖脂可能解释了为什么在这些物种的胰腺中未检测到PLRP2。
