Wang R, Ehara K, Fujita K, Tamaki N, Matsumoto S
Department of Neurosurgery, Kobe University, Japan.
No To Shinkei. 1991 Jul;43(7):649-55.
Spinal cord blood flow (SCBF) was measured simultaneously by laser Doppler flowmetry (LDF) and hydrogen clearance (HC) in the normal Wistar rats. Under normal physiological condition, SCBF values by HC was 45.0 +/- 1.7 ml/min/100 g at the level of Th-8. LDF values were well correlated (r = 0.78) to the SCBF measured by HC, where linear regression line was obtained as y = 14.2x - 1.58. Absolute SCBF values of LDF were also calculated as 43.7 +/- 1.3 mg/min/100 g using this equation. CO2 response of SCBF were 1.09 ml/min/100 g/mmHg by HC and 1.01 ml/min/100 g by LDF, respectively. Autoregulation of SCBF maintained at a range of 55 -155 mmHg of mean arterial pressure in both methods. In conclusion, LDF is a useful technique for continuous SCBF monitoring. Since absolute SCBF value in LDF has some variance, the relative value is more suitable for accurate evaluation of SCBF during time course of an experiment.
在正常Wistar大鼠中，通过激光多普勒血流仪（LDF）和氢清除法（HC）同时测量脊髓血流量（SCBF）。在正常生理条件下，在胸8水平，通过HC测得的SCBF值为45.0±1.7 ml/min/100 g。LDF值与通过HC测量的SCBF高度相关（r = 0.78），得到的线性回归线为y = 14.2x - 1.58。使用该方程计算出LDF的绝对SCBF值也为43.7±1.3 mg/min/100 g。通过HC和LDF测得的SCBF的CO2反应分别为1.09 ml/min/100 g/mmHg和1.01 ml/min/100 g。两种方法中，SCBF的自动调节均维持在平均动脉压55 - 155 mmHg的范围内。总之，LDF是一种用于连续监测SCBF的有用技术。由于LDF中的绝对SCBF值存在一定差异，相对值更适合在实验过程中准确评估SCBF。
