Watanabe Akihiko, Otake Remon, Nozaki Tetsuo, Morii Akihiro, Ogawa Ryohei, Fujimoto Shinichi, Nakamura Shinobu, Fuse Hideki, Kondo Takashi
Department of Urology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
Cancer Lett. 2008 Jun 28;265(1):107-12. doi: 10.1016/j.canlet.2008.02.004. Epub 2008 Mar 7.
We compared the enhancement effects of three different echo contrast agents (ECAs); Levovist, YM454, and MRX-815H as artificial microbubbles on ultrasound mediated gene transfection (USMGT) with 1MHz ultrasound at 0.2MPa using a luciferase expression vector in PC3 cells and elucidated the mechanisms of differences of USMGT facilitation by these ECAs. At a concentration of each ECA that induced iso-survival, ECAs with lipid shell (YM454 and MRX-815H) facilitated USMGT higher than those without shell (Levovist), and the order of the ECAs facilitating free radical formation by sonication was; YM454>MRX-815H>Levovist. These results suggested that the lipid shell type ECAs facilitated gene transfer higher than that by the non-shell type ECA.
