Effects of microbubbles on ultrasound-mediated gene transfer in human prostate cancer PC3 cells: comparison among Levovist, YM454, and MRX-815H.

作者信息

Watanabe Akihiko, Otake Remon, Nozaki Tetsuo, Morii Akihiro, Ogawa Ryohei, Fujimoto Shinichi, Nakamura Shinobu, Fuse Hideki, Kondo Takashi

机构信息

Department of Urology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.

出版信息

Cancer Lett. 2008 Jun 28;265(1):107-12. doi: 10.1016/j.canlet.2008.02.004. Epub 2008 Mar 7.

DOI:10.1016/j.canlet.2008.02.004

PMID:18329166

Abstract

We compared the enhancement effects of three different echo contrast agents (ECAs); Levovist, YM454, and MRX-815H as artificial microbubbles on ultrasound mediated gene transfection (USMGT) with 1MHz ultrasound at 0.2MPa using a luciferase expression vector in PC3 cells and elucidated the mechanisms of differences of USMGT facilitation by these ECAs. At a concentration of each ECA that induced iso-survival, ECAs with lipid shell (YM454 and MRX-815H) facilitated USMGT higher than those without shell (Levovist), and the order of the ECAs facilitating free radical formation by sonication was; YM454>MRX-815H>Levovist. These results suggested that the lipid shell type ECAs facilitated gene transfer higher than that by the non-shell type ECA.

摘要