Department of Medical Ultrasound, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, PR China.
Mol Med Rep. 2012 Apr;5(4):964-70. doi: 10.3892/mmr.2012.766. Epub 2012 Jan 25.
Non-invasive, efficient and tissue-specific transgenic technologies could be valuable in gene therapy. Although non-viral carriers may be safer and cheaper, they have a much lower transfection efficiency than viral gene carriers. The present study was designed to test the transgenic expression and safety of red fluorescent protein (RFP) in HeLa cells in vitro and in transplanted tumors of nude mice in vivo under ultrasound-mediated liposome microbubble destruction (UMLMD) conditions. Plasmids containing RFP were gently mixed with liposome microbubbles (LMs). The mixture was added to HeLa cells or injected into BALB/c mice by the tail vein under various ultrasound exposure and LM parameters, and then the transfection efficiencies were examined. The results in vivo and in vitro demonstrated that, following a comparison of the plasmid group, the ultrasound + plasmid group and the LM + plasmid group, UMLMD significantly increased the transgenic expression (P<0.01) without causing any apparent detrimental effect. From the study, we concluded that UMLMD could be a non-invasive, effective and promising non-viral technique for gene therapy and transgenic research.
非侵入性、高效且组织特异性的转基因技术在基因治疗中可能具有重要价值。虽然非病毒载体可能更安全、更便宜，但它们的转染效率比病毒基因载体低得多。本研究旨在测试在超声介导的脂质体微泡破坏（UMLMD）条件下，红色荧光蛋白（RFP）在体外 HeLa 细胞和体内裸鼠移植肿瘤中的转基因表达和安全性。将含有 RFP 的质粒与脂质体微泡（LM）轻轻混合。在各种超声暴露和 LM 参数下，通过尾静脉将混合物添加到 HeLa 细胞或 BALB/c 小鼠中，然后检查转染效率。体内和体外的结果表明，与质粒组、超声+质粒组和 LM+质粒组进行比较后，UMLMD 显著增加了转基因表达（P<0.01），而没有造成任何明显的不良影响。本研究得出结论，UMLMD 可能是一种非侵入性、高效且有前途的非病毒基因治疗和转基因研究技术。
