Vindal Vaibhav, Ashwantha Kumar Enjapoori, Ranjan Akash
Computational and Functional Genomics Group, Sun Centre of Excellence in Medical Bioinformatics, Centre for DNA Fingerprinting and Diagnostics, EMBnet India Node, Hyderabad 500 076, India.
FEBS Lett. 2008 Apr 2;582(7):1117-22. doi: 10.1016/j.febslet.2008.02.074. Epub 2008 Mar 7.
Mycobacterium tuberculosis harbors four mce operons. Among them, mce2 operon is preceded by a FadR-like regulator mce2R (Rv0586). Here, we report the operator sites of the mce2R and its orthologs in other sequenced mycobacteria and non-mycobacterial species Nocardia farciana. All the identified DNA motifs illustrate the FadR subfamily specific nucleotide preference. Moreover, these motifs from the upstream region share sequence conservation, which is in agreement with the similarity of their DNA binding domain. Using electrophoretic mobility shift assay, we demonstrate that the predicted DNA motifs specifically interact with the recombinant Mce2R-Rv0586. Our present study has implications in the understanding of cis-regulatory elements and the auto-regulatory nature of the FadR subfamily of regulators.
结核分枝杆菌含有四个mce操纵子。其中，mce2操纵子之前有一个类FadR调节因子mce2R（Rv0586）。在此，我们报告了mce2R及其在其他已测序分枝杆菌和非分枝杆菌物种法氏诺卡氏菌中的直系同源物的操纵子位点。所有鉴定出的DNA基序都显示出FadR亚家族特定的核苷酸偏好。此外，来自上游区域的这些基序具有序列保守性，这与其DNA结合结构域的相似性一致。通过电泳迁移率变动分析，我们证明预测的DNA基序与重组Mce2R-Rv0586特异性相互作用。我们目前的研究对于理解顺式调控元件以及FadR调节因子亚家族的自我调节性质具有重要意义。
