Gomes Brenda P F A, Montagner Francisco, Jacinto Rogério Castilho, Pinheiro Ericka Tavares, Zaia Alexandre A, Ferraz Caio Cezar Randi, Souza-Filho Francisco J
Endodontic Division, Department of Restorative Dentistry, Piracicaba Dental School, State University of Campinas, Piracicaba, Brazil.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008 Apr;105(4):519-25. doi: 10.1016/j.tripleo.2007.10.005.
To investigate the presence of Gemella morbillorum by culture or nested PCR in primary and secondary/persistent endodontic infections.
Microbial samples were taken from 50 cases with primary and 50 cases with secondary/persistent endodontic infections. Microbiologic techniques were used for culture and identification. The DNA extracted from the samples was analyzed for the presence of the target species using species-specific primers.
Culture and polymerase chain reaction (PCR) identified the species in 23 and 77, respectively, of 100 root canals. Culture yielded the test organism in 19 of 50 (38%) of root canal samples from primary and in 4 of 50 (8%) from secondary/persistent infections. PCR yielded the test organisms in 41 of 50 (82%) and 36 of 50 (72%) of the, respectively, primary and secondary/persistent root canal infections studied.
Gemella morbillorum was identified more frequently in primary endodontic infections than in secondary/persistent ones. A higher frequency of the target species was detected by PCR than by culture.
通过培养或巢式聚合酶链反应（PCR）研究麻疹孪生球菌在原发性和继发性/持续性牙髓感染中的存在情况。
从50例原发性和50例继发性/持续性牙髓感染患者中采集微生物样本。采用微生物学技术进行培养和鉴定。使用种特异性引物对从样本中提取的DNA进行分析，以检测目标菌种的存在情况。
在100个根管中，培养法和聚合酶链反应（PCR）分别鉴定出23个和77个含有该菌种。在50个原发性根管样本中，有19个（38%）培养出检测菌，在50个继发性/持续性感染根管样本中，有4个（8%）培养出检测菌。在所研究的原发性和继发性/持续性根管感染中，PCR分别在50个样本中的41个（82%）和36个（72%）中检测到检测菌。
麻疹孪生球菌在原发性牙髓感染中的检出率高于继发性/持续性牙髓感染。PCR检测目标菌种的频率高于培养法。
