麻疹孪生球菌在原发性和继发性/持续性牙髓感染中的情况

Gemella morbillorum in primary and secondary/persistent endodontic infections.

作者信息

Gomes Brenda P F A, Montagner Francisco, Jacinto Rogério Castilho, Pinheiro Ericka Tavares, Zaia Alexandre A, Ferraz Caio Cezar Randi, Souza-Filho Francisco J

机构信息

Endodontic Division, Department of Restorative Dentistry, Piracicaba Dental School, State University of Campinas, Piracicaba, Brazil.

出版信息

Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008 Apr;105(4):519-25. doi: 10.1016/j.tripleo.2007.10.005.

DOI:10.1016/j.tripleo.2007.10.005

PMID:18329586

Abstract

OBJECTIVE

To investigate the presence of Gemella morbillorum by culture or nested PCR in primary and secondary/persistent endodontic infections.

STUDY DESIGN

Microbial samples were taken from 50 cases with primary and 50 cases with secondary/persistent endodontic infections. Microbiologic techniques were used for culture and identification. The DNA extracted from the samples was analyzed for the presence of the target species using species-specific primers.

RESULTS

Culture and polymerase chain reaction (PCR) identified the species in 23 and 77, respectively, of 100 root canals. Culture yielded the test organism in 19 of 50 (38%) of root canal samples from primary and in 4 of 50 (8%) from secondary/persistent infections. PCR yielded the test organisms in 41 of 50 (82%) and 36 of 50 (72%) of the, respectively, primary and secondary/persistent root canal infections studied.

CONCLUSION

Gemella morbillorum was identified more frequently in primary endodontic infections than in secondary/persistent ones. A higher frequency of the target species was detected by PCR than by culture.

摘要

目的

通过培养或巢式聚合酶链反应（PCR）研究麻疹孪生球菌在原发性和继发性/持续性牙髓感染中的存在情况。

研究设计

从50例原发性和50例继发性/持续性牙髓感染患者中采集微生物样本。采用微生物学技术进行培养和鉴定。使用种特异性引物对从样本中提取的DNA进行分析，以检测目标菌种的存在情况。

结果

在100个根管中，培养法和聚合酶链反应（PCR）分别鉴定出23个和77个含有该菌种。在50个原发性根管样本中，有19个（38%）培养出检测菌，在50个继发性/持续性感染根管样本中，有4个（8%）培养出检测菌。在所研究的原发性和继发性/持续性根管感染中，PCR分别在50个样本中的41个（82%）和36个（72%）中检测到检测菌。