Department of Gastroenterology, Changhai Hospital, Naval Medical University, 168 Changhai Road, Shanghai, 200433, China.
The 92914th Military Hospital of PLA, Shanghai, China.
BMC Microbiol. 2018 Aug 29;18(1):92. doi: 10.1186/s12866-018-1232-6.
Colorectal cancer (CRC) is a common malignant gastrointestinal tumor. In China, CRC is the 5th most commonly diagnosed cancer. The vast majority of CRC cases are sporadic and evolve with the adenoma-carcinoma sequence. There is mounting evidence indicating that gut microbiota and inflammation play important roles in the development of CRC although study results are not entirely consistent. In the current study, we investigated the changes in the CRC-associated bacteria and plasma inflammatory factors and their relationships based on data from a case-control study of Han Chinese. We included 130 initially diagnosed CRC patients, 88 advanced colorectal adenoma patients (A-CRA), 62 patients with benign intestinal polyps and 130 controls.
Fecal microbiota composition was obtained using 16S ribosomal DNA (16S rDNA) sequencing. PCOA analysis showed structural differences in microbiota among the four study groups (P = 0.001, Unweighted Unifrac). Twenty-four CRC-associated bacteria were selected by a two-step statistical method and significant correlations were observed within these microbes. CRC-associated bacteria were found to change with the degree of malignancy. Plasma C-reactive protein (CRP) and soluble tumor necrosis factor II (sTNFR-II) displayed significant differences among the four study groups and increased with adenoma-carcinoma sequence. The correlations of CRP and sTNFR-II with several CRC-associated microbes were also explored.
CRC-associated species and plasma inflammatory factors tended to change along the adenoma-carcinoma sequence. Several CRC-associated bacteria were correlated with CRP and sTNFR-II. It is likely that gut microbiome and inflammation gradually form a microenvironment that is associated with CRC development.
结直肠癌（CRC）是一种常见的恶性胃肠道肿瘤。在中国，CRC 是第 5 大常见的癌症。绝大多数 CRC 病例为散发性，其发展遵循腺瘤-癌序列。越来越多的证据表明，肠道微生物群和炎症在 CRC 的发生发展中起着重要作用，尽管研究结果并不完全一致。在本研究中，我们基于汉族病例对照研究的数据，调查了 CRC 相关细菌和血浆炎症因子的变化及其相关性。我们纳入了 130 名初诊 CRC 患者、88 名高级结直肠腺瘤患者（A-CRA）、62 名良性肠息肉患者和 130 名对照。
采用 16S 核糖体 DNA（16S rDNA）测序获得粪便微生物群落组成。PCOA 分析显示，四组研究人群的微生物群落结构存在差异（P=0.001，非加权 UniFrac）。通过两步统计方法筛选出 24 种 CRC 相关细菌，这些微生物之间存在显著相关性。CRC 相关细菌的变化与恶性程度有关。四组研究人群的血浆 C 反应蛋白（CRP）和可溶性肿瘤坏死因子 II（sTNFR-II）均存在显著差异，且随着腺瘤-癌序列的进展而升高。还探讨了 CRP 和 sTNFR-II 与几种 CRC 相关微生物之间的相关性。
CRC 相关物种和血浆炎症因子倾向于沿着腺瘤-癌序列发生变化。几种 CRC 相关细菌与 CRP 和 sTNFR-II 相关。肠道微生物群和炎症可能逐渐形成与 CRC 发展相关的微环境。
