使用中空纤维支撑液膜萃取和液相色谱-质谱/质谱联用技术测定环境水体中的伊维菌素及其转化产物
Determination of ivermectin and transformation products in environmental waters using hollow fibre-supported liquid membrane extraction and liquid chromatography-mass spectrometry/mass spectrometry.
作者信息
Raich-Montiu J, Krogh K A, Granados M, Jönsson J A, Halling-Sørensen B
机构信息
Section of Toxicology and Environmental Chemistry, Department of Pharmaceutics and Analytical Chemistry, Faculty of Pharmaceutical Science, Copenhagen University, Universitetsparken 2, DK-2100 Copenhagen, Denmark.
出版信息
J Chromatogr A. 2008 Apr 11;1187(1-2):275-80. doi: 10.1016/j.chroma.2008.02.063. Epub 2008 Mar 10.
A simple and easy-to-use extraction method for aqueous samples based on hollow fibre-supported liquid membrane (HF-SLM) followed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) was developed to determine ivermectin and transformation products, the monosaccharide (TP1) and the aglycon of ivermectin (TP2). The proposed method attained enrichment factors up to 80, after optimising parameters, such as fibre length, organic solvent, stirring speed, salt level, pH in samples/fibre, extraction time and fibre emptying technique. Method validation with tap and lake water samples provided good linearity and detection limits of 0.2, 1.6 and 0.9 microg/l for ivermectin, TP1 and TP2 in lake water with RSD below 15%.
开发了一种基于中空纤维支撑液膜（HF-SLM）的简单易用的水样萃取方法，随后采用液相色谱-质谱/质谱（LC-MS/MS）来测定伊维菌素及其转化产物，即伊维菌素的单糖（TP1）和苷元（TP2）。在优化诸如纤维长度、有机溶剂、搅拌速度、盐浓度、样品/纤维中的pH值、萃取时间和纤维排空技术等参数后，该方法的富集因子高达80。对自来水和湖水样品进行方法验证，结果表明伊维菌素、TP1和TP2在湖水中具有良好的线性，检测限分别为0.2、1.6和0.9 μg/L，相对标准偏差低于15%。
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