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[土壤微生物对蛇足石杉扦插生根的影响机制]

[Mechanism of effects of soil microbes on cuttings rooting of Huperzia serrata].

作者信息

Li Na, Chen Jun, Zhu Da-Yuan

机构信息

College of Pharmacy, Jiangsu University, Zhenjiang 212013, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2007 Dec;32(23):2478-81.

PMID:18330236
Abstract

OBJECTIVE

To study the effects of soil microbes of habitats on the cuttings rooting of Huperzia serrata and its mechanism.

METHOD

The cuttings of H. serrata were planted on the primary habitat soil and on the soil autoclaved, rooting rates were examined respectively. Changes of contents of phenolic compounds (catechin, quercetin, chlorogenic acid, ferulic acid) and indoleacetic acid (IAA) , and changes of activities of polyphenoloxidase (PPO) and peroxydase (POD) during the cuttings rooting were determined.

RESULT

Soil microbes of primary habitat could increase the rooting rate and the number of roots, could increase contents of catechin, chlorogenic acid, ferulic acid and IAA during rooting, and could affect activities of PPO and POD.

CONCLUSION

During the rooting higher contents of catechin, chlorogenic, ferulic acid and IAA caused by soil microbes of primary habitat are important factor for rooting rate.

摘要

目的

研究不同生境土壤微生物对蛇足石杉扦插生根的影响及其机制。

方法

将蛇足石杉插穗分别接种于原生生境土壤和灭菌土壤上,分别测定其生根率。测定扦插生根过程中酚类化合物(儿茶素、槲皮素、绿原酸、阿魏酸)和吲哚乙酸(IAA)含量变化,以及多酚氧化酶(PPO)和过氧化物酶(POD)活性变化。

结果

原生生境土壤微生物能提高蛇足石杉扦插生根率和生根数,提高生根过程中儿茶素、绿原酸、阿魏酸和IAA含量,影响PPO和POD活性。

结论

原生生境土壤微生物引起的儿茶素、绿原酸、阿魏酸和IAA含量升高是提高扦插生根率的重要因素。

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