Gao Jie, Zeng Xiao-fei, Liu Xiao-hui, Yang Shang-xing
College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China.
Zhong Yao Cai. 2011 Jun;34(6):841-5.
To study the effects of different auxcin regulators on rooting of Periplocaforrestii cuttings as well as dynamic change rules of endogenous plant hormones and oxidases related to adventitious root formation.
Cuttings propagation characters of Periploca forrestii were investigated and compared with different concentration treatments of indolebutyric acid (IBA), Rooting Powder No. 1 (ABT1) and naphthylacetic acid (NAA). The dynamic changes of contents of endogenous hormones including indole acetic acid (IAA), abscisic acid (ABA), zeatin ribosides (ZRs) as well as the activities of indoleacetic acid oxidase( IAAO), polyphenol oxidase (PPO), peroxidase (POD) were tested.
Rooting percentage of cutting with 150 mg/L IBA, 150 mg/L ABT1 treatment and NAA treatment were 80% ,70% and 68% respectively, rooting percentage of cuttings of the control was 23% only. The adventitious rooting displayed three distinct phases i. e. root-inducing, root-formating and root-elongating phases. During root-inducing phase the contents of IAA, ABA and ZRs decreased,whereas IAAO activity kept at a higher level. The IAA content reached the peak and PPO activity increased obviously during root-formating phase, while activities of IAAO, POD and contents of ABA, ZRs declined to minimum. During root-elongating phase contents of IAA, ABA, ZRs were much steadfast and activities of PPO, IAAO, POD were increased. After that, the activities of the three oxidases decreased slowly. 150 mg/L IBA treatment increased the content of IAA and PPO activity in cuttings during rooting,while the opposite result occurred in contents of ZRs, ABA and the activities of IAAO, POD.
The dynamic changes of endogenous hormones (IAA, ABA, ZRs) contents and IAAO, PPO, POD activities are tightly related to the rooting process of cuttings in Periploca forrestii.
研究不同生长素调节剂对黑龙骨插穗生根的影响以及与不定根形成相关的内源植物激素和氧化酶的动态变化规律。
调查黑龙骨的扦插繁殖特性，并比较不同浓度吲哚丁酸（IBA）、1号生根粉（ABT1）和萘乙酸（NAA）处理的效果。测定内源激素吲哚乙酸（IAA）、脱落酸（ABA）、玉米素核苷（ZRs）含量以及吲哚乙酸氧化酶（IAAO）、多酚氧化酶（PPO）、过氧化物酶（POD）活性的动态变化。
150mg/L IBA、150mg/L ABT1处理和NAA处理的插穗生根率分别为80%、70%和68%，对照插穗生根率仅为23%。不定根形成表现为三个明显阶段，即诱根期、生根期和伸根期。在诱根期，IAA、ABA和ZRs含量下降，而IAAO活性保持在较高水平。在生根期，IAA含量达到峰值，PPO活性明显增加，而IAAO、POD活性以及ABA、ZRs含量降至最低。在伸根期，IAA、ABA、ZRs含量较为稳定；PPO、IAAO.POD活性增加，之后三种氧化酶活性缓慢下降。150mg/L IBA处理使插穗生根过程中IAA含量增加、PPO活性增强，而ZRs、ABA含量以及IAAO、POD活性则呈相反变化。
内源激素（IAA、ABA、ZRs）含量以及IAAO、PPO、POD活性的动态变化与黑龙骨插穗生根过程密切相关。
